FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564550
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38490.6817819502258435No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGA27920.49455318395182No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAACTTTTCCTAAC15950.28252590558852186No Hit
CATTTACACCAAGACATCATCAACAAATGTGAACAGTTTGTAGGCCCTCT13740.24337968293330972No Hit
GTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGGAT13390.23718005491099106No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC11460.20299353467363385No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11210.19856522894340628No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10800.19130280754583298No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAACACATAGCGCC10720.18988574971216013No Hit
CCTTTAGATAAGGGTATTAAACCTTACTATCCAGAACATCTAGTTAATCA10270.1819147993977504No Hit
GTTTAATACCCTTATCTAAAGGTAAATATTTGGTAAAGTTAGGGTAAAAC10080.17854928704277745No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAACTTTTC9950.17624656806305908No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTTCAAACC9490.16809848551944026No Hit
GAGTAACCCCATCTTTTCGTCTTGTTAGGATTTAAATGTATGCCCAAAGA8770.15534496501638473No Hit
GTCCAAGAGTCCTCTTATGTAAGATCTTGGGCAAGATTTGGTGGGCGTTC8080.14312284120095653No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7950.14082012222123816No Hit
GTTTTAGAAAACTCCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA7940.14064298999202904No Hit
ATCTAAAGGTAAATATTTGGTAAAGTTAGGGTAAAACCTAGCAGGCATAA7870.1394030643875653No Hit
CTATTTACACACTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAA7680.13603755203259232No Hit
TTCTAAAACAGTCCTTCAGCTTGTGTAGGATGTGCTCTTGTGGCAATGAT7660.1356832875741741No Hit
CCTACAAACTGTTCACATTTGTTGATGATGTCTTGGTGTAAATGTATGTT7640.1353290231157559No Hit
GTCTTTAACCCTCATTGGAAAACACCAACTTTTCCTAACATACATTTACA7560.13391196528208307No Hit
AGATAAGGGTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACT7520.13320343636524665No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGATTTCTTTCCGTCTGTACG7470.13231777521920113No Hit
CTCTTACTGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGG7370.1305464529271101No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT7000.12399256044637322No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6840.12115844477902755No Hit
GGTAAATATTTGGTAAAGTTAGGGTAAAACCTAGCAGGCATAATCAATTG6800.12044991586219113No Hit
GCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTTGACATACT6730.11920999025772738No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGGATAGTAAGGTTTAATAC6720.11903285802851828No Hit
GGGTAAAGGTTCATGTATTGTTTACATAGAAAGGCCTTGTACGTTGGCGA6640.11761580019484545No Hit
ATCCAGAACATCTAGTTAATCATTACTTTCAAACCAGACACTATTTACAC6630.11743866796563635No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAA6490.1149588167567089No Hit
GTCTAGAAGATCTCGTACAGACGGAAAGAAATCAGAAGGCAAAAATGAGA6430.11389602338145427No Hit
GTTCTGGATAGTAAGGTTTAATACCCTTATCTAAAGGTAAATATTTGGTA6310.11177043663094499No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6220.11017624656806306No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTTCAAACCAGACACTATTTACACACTCTATGGA6210.10999911433885395No Hit
GTTTGTAGGCCCTCTTACTGTCAATGAGAAAAGAAGATTGCAATTGATTA6200.10982198210964485No Hit
ATATTTACCTTTAGATAAGGGTATTAAACCTTACTATCCAGAACATCTAG6170.10929058542201754No Hit
CAGTAAGAGGGCCTACAAACTGTTCACATTTGTTGATGATGTCTTGGTGT6090.1078735275883447No Hit
CTTATATAAAATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTT6040.10698786644229917No Hit
CTTCTAGACACCGCCGCAGCTCTATATCGGGATGCGTTAGAGTCTCCTGA6030.10681073421309006No Hit
CTCTTATATAAAATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGT5830.10326808962890799No Hit
GTCAACAACCGATCTTGAGCAATACTTCAAAGACTGTTTGTTTAAGGACT5780.10238242848286247No Hit
ATCCTACACAAGCTGAAGGACTGTTTTAGAAAACTCCCTGTTAACAGGCC5750.10185103179523514No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAA5740.10167389956602603No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGGGC5700.10096537064918962No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTGACAGTAAGAGGGCCTAC5660.10025684173235319No Hit
CATCCTACACAAGCTGAAGGACTGTTTTAGAAAACTCCCTGTTAACAGGC5660.10025684173235319No Hit
CCCTTATCTAAAGGTAAATATTTGGTAAAGTTAGGGTAAAACCTAGCAGG5650.1000797095031441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)