FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477865
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28750.6016343527983845No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17870.37395498728720455No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13660.28585479162524985No Hit
CATCTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11340.23730551515595408No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCCAATACAAAGACC10800.2260052525294801No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10450.2186810082345432No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10280.21512351814843103No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9270.1939878417544704No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9190.19231372877277056No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG8760.18331537149613386No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC8690.18185052263714646No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8690.18185052263714646No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8640.18080420202358408No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8200.17159658062423488No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8080.1690854111516851No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7770.1625982233475982No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7720.16155190273403575No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7550.15799441264792358No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACATTCTTTGATTTTCTGT7520.15736662027978612No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT7330.153390601948249No Hit
GTCTTACATAAGAGGACTCTTGGACTCTATGCAATGTCAACGACCGACCT7180.15025164010756176No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7100.1485775271258619No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6880.1439737164261873No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6870.14376445230347484No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGAAATTGGATCTTC6470.13539388739497557No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6200.12974375608173855No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6160.12890669959088863No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6130.1282789072227512No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6020.1259770018729139No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6010.12576773775020142No Hit
GTATACATCTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT5840.12221024766408922No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5790.12116392705052682No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5660.11844349345526456No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5660.11844349345526456No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5650.11823422933255208No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5620.11760643696441464No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA5620.11760643696441464No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5610.11739717284170216No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT5510.11530453161457734No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5460.11425821100101492No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCATAGAGTCCAA5350.11195630565117765No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5330.11153777740575267No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5250.10986366442405282No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5240.10965440030134034No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5070.10609691021522816No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4950.10358574074267837No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4850.10149309951555355No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCATTGAC4810.10065604302470363No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4800.10044677890199115No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4780.10002825065656619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)