FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509517
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGG37240.7308882726189705No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT25950.5093058720317477No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC24610.48300645513299856No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24450.47986622624956576No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT20950.4111737194244745No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT19570.38408924530486716No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT16760.3289389755395796No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT16450.3228547820779287No Hit
GGATAATAAGGTTTAATCCCCTTATCTAATGGCAAATATTTGGTAACATT16110.31618179570063415No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15770.3095088093233396No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGG15070.2957703079583213No Hit
ATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGA14070.27614387743686664No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC13990.2745737629951503No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12720.24964819623290294No Hit
TAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGT12720.24964819623290294No Hit
CACTAATATGGGCCTGAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11650.22864791557494646No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGGATTAAACCTTAT11560.22688153682801554No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA11360.2229562507237246No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT11150.21883470031421914No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT11030.2164795286516446No Hit
AGATAAGGGGATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT10550.20705884200134636No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG10520.20647004908570274No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG10500.20607752047527364No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC10380.2037223488126991No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA10210.20038585562405178No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC9830.19292781202589904No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGA9690.1901801117528954No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG9660.18959131883725175No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC9600.18841373300596448No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA9580.18802120439553538No Hit
GTGTAAACGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9560.1876286757851063No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9500.186451089953819No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCTGTACG9430.1850772398173172No Hit
TTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACCAGACATTATT9240.1813482180182408No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT9010.17683413899830625No Hit
CTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACACC8770.1721237956731571No Hit
CCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGT8500.16682465943236438No Hit
GACATATCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG8470.16623586651672073No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCCAGAAGATCTCGTA8390.16466575207500436No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8270.1623105804124298No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACA8150.15995540874985525No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA8110.15917035152899708No Hit
GTTCTGGATAATAAGGTTTAATCCCCTTATCTAATGGCAAATATTTGGTA8070.15838529430813889No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCT7990.15681517986642252No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC7900.1550488011194916No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGGATTAAAC7850.15406747959341888No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC7820.15347868667777523No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7790.1528898937621316No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG7740.15190857223605886No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7740.15190857223605886No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC7550.14817955043698247No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG7450.146216907384837No Hit
TCCCGATACAGAGCTGAGGCGGTATCCAGAAGATCTCGTACAGAAGGAAA7410.14543185016397883No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG7400.14523558585876428No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC7330.14386173572226246No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7290.14307667850140426No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC7220.14170282836490244No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7110.13954392100754243No Hit
TAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTA7020.1377775422606115No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6900.13542237059803697No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCT6900.13542237059803697No Hit
ATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGAAG6850.13444104907196422No Hit
ATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT6780.1330671989354624No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6730.13208587740938968No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA6700.13149708449374603No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6670.1309082915781024No Hit
ATGATGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGATACAGAG6630.1301232343572442No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6610.12973070574681514No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6550.12855311991552784No Hit
GGATATGTCATTGGATGTTATGGGTCACTGCCACAAGATCACATCATTCA6490.12737553408424057No Hit
TACCCACATTGTGTAAACGGGGCAGCAAAACCCAAAAGACCCACAATACG6480.12717926977902602No Hit
GGTTTAATCCCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA6460.12678674116859692No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6430.1261979482529533No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6270.12305771936952055No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA6160.12089881201216054No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6150.120702547706946No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6140.12050628340173145No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6110.1199174904860878No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT6110.1199174904860878No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6050.11873990465480053No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5940.11658099729744051No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT5900.11579594007658234No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5890.11559967577136779No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5810.11402956132965142No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTGAAGTT5770.11324450410879322No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA5760.11304823980357867No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5720.1122631825827205No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGA5710.11206691827750595No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5650.11088933244621868No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5590.1097117466149314No Hit
GTTATGTCAACACTAATATGGGCCTGAAGTTCAGGCAACTATTGTGGTTT5510.10814163217321503No Hit
CCTACAAATTGTTCACATTTTTTGATGATGTCTTGGTGTAAATGTATATT5450.10696404634192776No Hit
CCTCATCATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAAT5400.10598272481585501No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5380.10559019620542594No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5340.10480513898456774No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC5330.10460887467935319No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5330.10460887467935319No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5300.10402008176370954No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5280.10362755315328047No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5270.10343128884806592No Hit
ATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGT5210.10225370301677862No Hit
TCATCATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAATGA5190.10186117440634955No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5190.10186117440634955No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG5170.10146864579592045No Hit
ACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATAT5100.10009479565941863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)