FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509517
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGG36310.7126356922340177No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24390.4786886404182785No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT23470.4606323243385402No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT21990.4315852071667874No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21850.4288375068937837No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT21750.42687486384163825No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGG18710.36721051505641616No Hit
GGATAATAAGGTTTAATCCCCTTATCTAATGGCAAATATTTGGTAACATT17230.3381633978846633No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15610.30636858043990683No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT15600.30617231613469226No Hit
ATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGA15520.3046022016929759No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT14320.28105048506723035No Hit
TAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGT13580.2665269264813539No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC13070.25651744691541206No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG12830.2518071035902629No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA11670.22904044418537556No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11650.22864791557494646No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA11610.22786285835408826No Hit
CACTAATATGGGCCTGAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11000.21589073573600095No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10880.21353556407342641No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGGATTAAACCTTAT10660.20921774935870638No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCTGTACG10160.19940453409797906No Hit
AGATAAGGGGATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT9970.1956755122989027No Hit
GTGTAAACGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9780.19194649049982632No Hit
CCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGT9660.18959131883725175No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCT9550.18743241147989176No Hit
GTTCTGGATAATAAGGTTTAATCCCCTTATCTAATGGCAAATATTTGGTA9250.18154448232345535No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC8920.17506776025137533No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT8800.17271258858880076No Hit
TTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACCAGACATTATT8740.1715350027575135No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA8700.1707499455366553No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTT8660.16996488831579712No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGA8380.1644694877697898No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG8290.1627031090228589No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGGATTAAAC8150.15995540874985525No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA8040.15779650139249526No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8020.15740397278206616No Hit
CTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACACC7880.1546562725090625No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7870.15446000820384798No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7780.15269362945691706No Hit
ATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT7780.15269362945691706No Hit
GGTTTAATCCCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA7700.1511235150152007No Hit
ATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGAAG7680.1507309864047716No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACA7540.14798328613176792No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7460.14641317169005155No Hit
GGATATGTCATTGGATGTTATGGGTCACTGCCACAAGATCACATCATTCA7370.14464679294312063No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7370.14464679294312063No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7360.14445052863790608No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGA7340.144058000027477No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7260.14248788558576062No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA7200.14131029975447335No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC7160.14052524253361517No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC7120.13974018531275698No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG7090.13915139239711333No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC7010.13758127795539696No Hit
GACATATCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG6980.13699248503975334No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC6960.13659995642932424No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6960.13659995642932424No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG6910.1356186349032515No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6890.13522610629282242No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6880.13502984198760787No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6870.13483357768239332No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6820.1338522561563206No Hit
TAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTA6750.13247840601981878No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6740.13228214171460423No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6690.1313008201885315No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6460.12678674116859692No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6450.1265904768633824No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6430.1261979482529533No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6420.12600168394773875No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6420.12600168394773875No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG6310.12384277659037873No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA6290.12345024797994963No Hit
ATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAA6250.12266519075909146No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6250.12266519075909146No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCT6220.12207639784344781No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA6220.12207639784344781No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC6200.12168386923301872No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6120.12011375479130235No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5990.11756231882351326No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC5980.11736605451829871No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCCAGAAGATCTCGTA5900.11579594007658234No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5880.11540341146615324No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5850.11481461855050959No Hit
CCTACAAATTGTTCACATTTTTTGATGATGTCTTGGTGTAAATGTATATT5770.11324450410879322No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTG5740.1126557111931496No Hit
ATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTACAAATTG5740.1126557111931496No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAA5710.11206691827750595No Hit
ATGTGGGTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGC5700.1118706539722914No Hit
TCCCGATACAGAGCTGAGGCGGTATCCAGAAGATCTCGTACAGAAGGAAA5680.1114781253618623No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5640.11069306814100413No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5520.10833789647842958No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT5510.10814163217321503No Hit
ATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA5490.10774910356278593No Hit
GTTATGTCAACACTAATATGGGCCTGAAGTTCAGGCAACTATTGTGGTTT5430.10657151773149866No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5410.10617898912106956No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5390.10578646051064046No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5270.10343128884806592No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5270.10343128884806592No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5210.10225370301677862No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5190.10186117440634955No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5170.10146864579592045No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTAC5130.10068358857506225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)