FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469651
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTAGAACAGGGCTGAGAGTTCCTGAACTGGAGCCACCAGCAGGAAAAT32310.6879576536619745No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23160.49313213428694924No Hit
GTTCCAACTATTGCCTCTCACATCTCGTCAATCTCCTCGAGGATTGGGGA23090.49164166583271407No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT22340.4756723609659087No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT18420.39220612752873946No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA17540.3734688098183545No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA17230.36686816380674164No Hit
GTTCTAACTATTGCCTCTCACATCTCGTCAATCTCCTCGAGGATTGGGGA15610.33237446529444203No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA14830.3157663882329645No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14830.3157663882329645No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG14420.3070365015724442No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13470.2868087154078241No Hit
GTCCCCATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA12900.274672043709052No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG12670.2697747902165651No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT12270.2612578276209355No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12100.257638118517793No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC11600.24699191527325612No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG10980.23379062325003036No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG10960.23336477512024886No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT10880.23166138260112298No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT10750.22889336975754335No Hit
AGTTGGAACAGGGCTGAGAGTTCCTGAACTGGAGCCACCAGCAGGAAAAT10680.2274029013033082No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG10340.2201634830970231No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9920.2112206723716121No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG9870.21015605204715843No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA9700.2065363429440159No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA9700.2065363429440159No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCAGACATTATT9250.19695476002393264No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9200.19589013969947897No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8680.1848180883251606No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA8370.17821744231354772No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG8190.17438480914551444No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC8180.1741718850806237No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG7980.16991340378280895No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC7850.16714539093922934No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7810.1662936946796664No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC7790.1658678465498849No Hit
GATAGGGACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAG7780.1656549224849942No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA7560.16097059305739794No Hit
GTATGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTACATGATCAGG7500.1596930486680535No Hit
GTGCCAGCAGTGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA7460.15884135240849057No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7380.15713795988936466No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7340.1562862636298017No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT7320.15586041550002022No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA7320.15586041550002022No Hit
CCTATTAGCAATATCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT7310.1556474914351295No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGA7250.15436994704578505No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGAAAGTAATGATTAACTACA6970.1484080732288444No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6850.14585298445015554No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG6540.13925233843854265No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT6430.13691017372474454No Hit
CATGAAGATATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT6420.1366972496598538No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6370.13563262933540013No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT6280.13371631275138346No Hit
ACTAGATACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC6270.13350338868649275No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA6260.133290464621602No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6140.13073537584291314No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5910.12583812235042616No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5870.12498642609086322No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA5840.12434765389619098No Hit
CTGCCGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCC5820.12392180576640953No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5810.12370888170151878No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5790.12328303357173731No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA5750.12243133731217437No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT5650.12030209666326697No Hit
ATACAGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTAATGACTCTA5620.11966332446859476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5590.11902455227392256No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC5550.11817285601435959No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT5510.11732115975479664No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5480.11668238756012443No Hit
GCACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG5480.11668238756012443No Hit
GGGTTATTCCCTGAATTTCATGGGGTATGTAATTGGAAGTTGGGGTACAT5440.11583069130056149No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC5410.11519191910588926No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC5360.11412729878143557No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5310.11306267845698188No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATATACTGTTTACTT5270.11221098219741893No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC5180.1102946656134023No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5160.10986881748362082No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCATTT5090.10837834902938566No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT5040.10731372870493197No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC5040.10731372870493197No Hit
GTGCTGTATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGA5000.106462032445369No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG4970.1058232602506968No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4910.10454571586135236No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG4850.10326817147200795No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT4850.10326817147200795No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC4810.10241647521244499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)