FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575731
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTAACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGG24390.42363534358928046No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23780.41304011769385357No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG22310.38750736020815274No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA17500.3039613986392951No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATGAACTAGA16720.29041340487137224No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG16590.28815540591005173No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT16000.27790756447021264No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14950.25966988055185497No Hit
CTTTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT14910.25897511164067943No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAC13760.2390005054443829No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATGAACTAGATGTTCTGGAT13750.238826813216589No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT13650.23708989093865016No Hit
ATACTAATGTGGGCCTAAAGTTCAGGCAACTACTGTGGTTTCACATTTCT12770.22180497489278847No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC11800.20495682879678184No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCCGTGGA11060.19210360394003448No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT10690.1856769915116608No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT10530.1828979158669587No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT10340.1795977635388749No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT10220.17751345680534833No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC10070.17490807338844008No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA10020.17403961224947068No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT9930.17247638219932573No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9870.17143422883256243No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA9820.17056576769359302No Hit
TGATGAACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGG9480.164660231948601No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC9430.1637917708096316No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA9420.16361807858183772No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9400.16327069412624995No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC9310.161707464076105No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA9220.16014423402596004No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATTCAGAGAGTAACCCCA9220.16014423402596004No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC9020.15667038947008238No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA8870.15406500605317414No Hit
CCCAAAACATCCAATGACATAGCCCATAAAATTCAGAGAGTAACCCCATC8860.15389131382538027No Hit
AAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAAT8850.1537176215975864No Hit
CCTCCAGCTTTTAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8660.15041746926950259No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT8650.15024377704170871No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT8410.14607516357465553No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8330.14468562575230445No Hit
CTTTCCAATCAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTG8050.13982224337407576No Hit
CTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT7590.13183240089555714No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7500.1302691708454122No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7470.12974809416203054No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCATTAAC7400.12853224856747336No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7210.12523209623938958No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT7170.12453732732821406No Hit
GTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACA7030.1221056361390997No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6970.12106348277233639No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATGTT6920.12019502163336697No Hit
AGTTCATCATTACTTCCAAACTAGACACTATTTACACACTCTATGGCAGG6840.1188054838110159No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTTTTATA6770.11758963821645874No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6620.11498425479955048No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6570.11411579366058108No Hit
CCCATAACATCCAATGACATAGCCCATAAAATTCAGAGAGTAACCCCATC6490.11272625583823No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6430.1116841024714667No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT6250.10855764237117681No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT6080.1056048744986808No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG5890.10230472217059702No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5880.10213102994280315No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5860.10178364548721539No Hit
CTACCACTGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAGT5760.10004672320927654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)