FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763808
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65720.8604256567095396No Hit
ACTACATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATA41890.5484362562319326No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT30130.39447086178725543No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA30130.39447086178725543No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28560.3739159579370732No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT21370.2797823536805061No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC20950.27428358959319615No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT20640.27022497800494366No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT19430.25438330051531277No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG18930.24783715279232477No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT18540.24273115756839417No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC17030.22296179144497044No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT16430.2151064141773849No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT16130.2111787255435921No Hit
ACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATA15470.20253781054924797No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT15430.20201411873140893No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA15080.19743181532531737No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA14140.18512505760609999No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG13780.1804118312455486No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT13270.1737347605681009No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT12840.16810507352633122No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC12530.16404646193807867No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC12470.1632609242113201No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT12460.16313000125686036No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT12240.16024969625874566No Hit
CTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA12170.15933323557752735No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC12100.158416774896309No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT11780.15422724035359672No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT11770.15409631739913696No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG11520.15082324353764298No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGT11440.14977585990196488No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11100.14532447945033308No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11070.14493171058695378No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10930.14309878922451716No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10700.1400875612719427No Hit
ACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA10570.1383855628639658No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10450.1368144874104487No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC10400.1361598726381499No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA10100.13223218400435713No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC10080.1319703380954376No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGGAT10060.1317084921865181No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9950.1302683396874607No Hit
GTATGATGTGATCTTGTGGTAATGACCCATAACATCCAATGACGTAGCCC9900.12961372491516193No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA9710.12712618878042647No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT9590.12555511332690938No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT9570.12529326741798985No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9560.1251623444635301No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA9470.12398403787339225No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC9410.12319850014663372No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9190.12031819514851899No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA9140.11966358037622021No Hit
ACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTAT9070.11874711969500189No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9070.11874711969500189No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8960.11730696719594452No Hit
CTCTTTACATTTCATGGGCTACGTCATTGGATGTTATGGGTCATTACCAC8650.11324835560769199No Hit
CCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG8640.11311743265323222No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC8600.11259374083539318No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC8570.11220097197201391No Hit
AGACTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8410.11010620470065775No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT8360.10945158992835896No Hit
CTCTATGACGGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8310.10879697515606016No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC8300.10866605220160039No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT8120.10630943902132473No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCT8100.10604759311240522No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC8020.10500020947672713No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA8010.10486928652226739No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA7940.10395282584104906No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC7840.10264359629645145No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC7830.10251267334199171No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7830.10251267334199171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)