FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535778
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT27030.5044999981335553No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT25810.4817293729865728No Hit
CATTTAAACCCTACCAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT21890.40856474136676013No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA20060.37440880364628637No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCCATGAA16980.31692230737357635No Hit
CCTATTAGCAATGTCTTCATGCAAATGAATTTTAGGAAAAGAAGGTGTTT15690.2928451709476686No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG15290.28537939221095304No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT15080.28145985837417736No Hit
CTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCATTTGCATG14540.2713810570796113No Hit
GTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATG14290.26671494536916407No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA13560.2530898991746582No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA13490.25178338789573296No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTC12810.23909156404331644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12760.238158341701227No Hit
CTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTGTCCCTATC12470.23274565211710818No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT12100.2258398067856463No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG11750.21930725039102017No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG11660.21762745017525914No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT11230.2096017380332899No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTG10650.19877635886505232No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10580.1974698475861271No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10380.1937369582177693No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9970.18608453501263583No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA9940.18552460160738216No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCA9670.18048520096009915No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9430.1760057337180698No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC9290.17339271116021934No Hit
ACTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTT9110.17003311072869734No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC9010.16816666604451844No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGAA8720.16275397646039963No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG8270.15435497538159462No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTC8190.1528618196342515No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT8170.15248853069741572No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC8020.14968886367114737No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC7790.1453960408975359No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC7670.14315630727652126No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT7640.14259637387126758No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA7640.14259637387126758No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCT7610.1420364404660139No Hit
GAATTAATGACTCTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCAGC7470.13942341790816346No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7240.135130595134552No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7090.13233092810828367No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT6920.12915797214517954No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT6790.12673159405574697No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA6740.12579837171365751No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG6470.12075897106637451No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT6440.12019903766112083No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGA6240.11646614829276304No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6180.11534628148225572No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT6120.11422641467174836No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC6110.11403977020333048No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGC6070.11329319232965894No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG6040.11273325892440526No Hit
GTTTCTCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATG6020.11235996998756947No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT5950.11105345870864425No Hit
GGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTT5910.1103068808349727No Hit
TTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTT5880.10974694742971902No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5860.10937365849288325No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA5760.10750721380870436No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA5750.10732056934028647No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA5740.10713392487186858No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5710.1065739914666149No Hit
CTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAA5670.10582741359294334No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA5500.10265445762983921No Hit
ATTACATACCCCATGAAATTCAGGGAATAACCCCATCTCTTTGTTTTGGT5480.10228116869300345No Hit
GCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGAT5470.10209452422458556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)