FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535778
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT25420.47445023871827513No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT23610.4406675899346371No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA18580.3467854223204387No Hit
CATTTAAACCCTACCAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT18150.33875971017846945No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG17610.3286809088839034No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCCATGAA17060.3184154631209195No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT15400.2874324813635498No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT14200.2650351451534031No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG14110.26335534493764207No Hit
GTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATG13860.25868923322719484No Hit
CTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCATTTGCATG13090.24431760915901735No Hit
CCTATTAGCAATGTCTTCATGCAAATGAATTTTAGGAAAAGAAGGTGTTT12900.24077136425907747No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA12890.24058471979065957No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12730.23759840829597334No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG12550.2342388078644513No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTC12230.22826618487507885No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT11960.22322678422779582No Hit
CTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTGTCCCTATC11360.21202811612272246No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTG11320.2112815382490509No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAAATTCA11060.20642878207018578No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA10680.199336292270306No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA10640.19858971439663445No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT10600.19784313652296287No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC9610.1793653341495918No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9430.1760057337180698No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8830.16480706561299643No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC8790.16406048773932486No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8740.1631272653972354No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8710.16256733199198176No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC8290.1547282643184304No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA8150.15211524176057994No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT7900.14744913005013272No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG7740.14446281855544646No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC7680.14334295174493913No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCT7570.14128986259234233No Hit
ACTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTT7570.14128986259234233No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGAA7450.13905012897132768No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT7420.138490195566074No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTC7380.13774361769240245No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG7370.13755697322398458No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT7190.13419737279246255No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG7120.13289086151353732No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7030.13121106129777632No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6670.12449186043473229No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC6520.12169219340846395No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT6360.11870588191377773No Hit
GAATTAATGACTCTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCAGC6110.11403977020333048No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT6020.11235996998756947No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC5950.11105345870864425No Hit
GTTTCTCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATG5850.10918701402446536No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGA5810.1084404361507938No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA5800.1082537916823759No Hit
CTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTTTTCCTAAA5700.10638734699819702No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA5680.10601405806136123No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT5660.10564076912452545No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT5600.10452090231401813No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA5570.10396096890876445No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA5520.103027746566675No Hit
TTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACACCTTCTT5470.10209452422458556No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA5450.10172123528774978No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5420.10116130188249611No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCGAT5410.10097465741407821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)