FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321637
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT15750.4896824681240032No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT11050.3435550014457292No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA10240.3183713316564947No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG10220.31774951264935314No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9160.28479310527084883No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9080.2823058292422824No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT9000.27981855321371607No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG8660.269247630092309No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA8450.2627185305173223No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT8310.2583657974673312No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8130.2527694264030569No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7960.24748396484235335No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC7780.241887593778079No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT7780.241887593778079No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT7770.24157668427450824No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG7740.24064395576379582No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT6930.2154602859745614No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6930.2154602859745614No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG6810.21172937193171185No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA6660.20706572937814993No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT6370.19804935377459681No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT5830.18126024058177384No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT5770.17939478356034907No Hit
GAATTACAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAAT5740.1784620550496367No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA5660.17597477902107034No Hit
GGGGTATATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG5370.16695840341751728No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5200.16167294185681372No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG5030.15638748029611022No Hit
GTCAGTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA5000.15545475178539783No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4970.15452202327468542No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4830.1501692902246943No Hit
ATATACCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG4780.1486147427068403No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT4600.14301837164256598No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG4570.1420856431318536No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4560.14177473362828283No Hit
GGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG4550.141463824124712No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG4540.1411529146211412No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC4530.14084200511757045No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG4500.13990927660685804No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA4440.13804381958543327No Hit
CTGACATACTTTCCAATCAATTGGTCGATTGACAGGAAGTTTTCTAAAAC4360.1355565435568669No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4280.13306926752830053No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC4270.13275835802472974No Hit
ATTGACCACAGTGCCAGCAGTGCCACCTCCTGCCTCCACCAATCGGCAGT4250.13213653901758815No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4200.13058199149973418No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACTTTCCACCAAGCTCTGCAAG4190.13027108199616338No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTCTGCGGGTCACCATA4160.12933835348545097No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTTTCCATGC4090.12716198696045541No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA4080.12685107745688462No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3870.12032197788189791No Hit
CCTCTACTTCCAGGATCAACAACAACCAGCACGGGACCCTGCAAAACCTG3830.11907833986761474No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3820.11876743036404393No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT3800.11814561135690235No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3780.11752379234976076No Hit
CATTCTGCGGGTCACCATATTCTTGGGAACAAGAGCTACATCATGGGAGG3740.11628015433547756No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG3670.11410378781048201No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT3620.11254924029262803No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3620.11254924029262803No Hit
GAGCAATGCTCAGGAGACTCTAAGGCTTCCCGATATAGAGCTGAGGCTGT3560.11068378327120323No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT3560.11068378327120323No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA3550.11037287376763245No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC3470.10788559773906609No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3430.1066419597247829No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT3420.10633105022121211No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT3360.10446559319978735No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC3360.10446559319978735No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT3320.10322195518550414No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG3290.10228922667479176No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT3290.10228922667479176No Hit
TCCTAATACAAAGTGGTCTGACCTAATCTCCTCCCCCAACTCCTCCCAGT3270.10166740766765017No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA3250.1010455886605086No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGAT3240.10073467915693779No Hit
GGCAAGACCTGATGGGCGTTCACGGTGGTTTCCATGCAACGTGCAGAGGT3220.10011286014979619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)