FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321637
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT14520.4514405991847953No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT10460.3252113407350522No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG10210.31743860314578237No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA9480.2947422093851143No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9440.2934985713708311No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9190.2857258337815612No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8880.27608763917086654No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8760.272356725128017No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG8640.2686258110851675No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC8330.25898761647447277No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA8170.25401306441734006No Hit
GGGGTATATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG7890.24530759831735777No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT7680.23877849874237106No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG7560.23504758469952153No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT7370.22914030413167638No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT7130.22167847604597732No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT7070.2198130190245525No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6990.21732574299598617No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT6890.2142166479602782No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT6750.20986391491028705No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6370.19804935377459681No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT6200.1927638922138933No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6030.18747843065318978No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA5980.18592388313533578No Hit
ATATACCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG5920.18405842611391102No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG5770.17939478356034907No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG5400.16789113192822966No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT5280.1641602178853801No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5190.16136203235324295No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG5140.15980748483538895No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC5030.15638748029611022No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG4960.15421111377111463No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA4910.15265656625326066No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4880.15172383774254827No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTCTGCGGGTCACCATA4880.15172383774254827No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA4800.14923656171398192No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4800.14923656171398192No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4720.14674928568541554No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4530.14084200511757045No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4490.13959836710328724No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA4470.13897654809614565No Hit
GAATTACAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAAT4420.13742200057829168No Hit
GGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG4180.12996017249259256No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACTTTCCACCAAGCTCTGCAAG4170.1296492629890218No Hit
GTCAGTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA4000.12436380142831825No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA3940.12249834440689349No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3890.1209437968890395No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC3870.12032197788189791No Hit
GGGATCACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATC3810.11845652086047315No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA3780.11752379234976076No Hit
CCTCTACTTCCAGGATCAACAACAACCAGCACGGGACCCTGCAAAACCTG3730.11596924483190678No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT3710.11534742582476518No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT3630.11286014979619882No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT3570.11099469277477406No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCAATTGATTGGAAAGTATGTCAG3570.11099469277477406No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT3550.11037287376763245No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA3540.11006196426406167No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC3540.11006196426406167No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3500.10881832624977847No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGAT3360.10446559319978735No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG3350.10415468369621653No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3340.10384377419264575No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT3340.10384377419264575No Hit
ATTGACCACAGTGCCAGCAGTGCCACCTCCTGCCTCCACCAATCGGCAGT3330.10353286468907497No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG3330.10353286468907497No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA3310.10291104568193336No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG3310.10291104568193336No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC3270.10166740766765017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)