FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416239
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG34630.8319739380500145No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTTGACAGACT28690.689267464125178No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT26840.6448218451418536No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT24670.5926883353073594No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23190.5571318401206998No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACATTTCAT21390.5138874540828706No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT19780.4752077532379234No Hit
GTTTTGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTC18140.435807312625679No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17890.4298011478982027No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC16080.3863165152712744No Hit
GCCGTGCAACGGGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTG15940.3829530630238877No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG14980.3598893904703788No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT14510.3485978007827234No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC14160.3401891701642566No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG14130.33946843039695945No Hit
CAATAGGCCTGTTAGCAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT13810.3317805395457898No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCT13530.32505363505101637No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13230.31784623737804485No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC12870.309197360170479No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT12870.309197360170479No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT12610.30295094885390367No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC12590.3024704556757055No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA11850.2846922080823757No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC11610.27892628994399854No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11380.27340061839472035No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA11180.2685956866127393No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11070.26595297413264973No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA10840.26042730258337154No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG10730.25778459010328203No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC10340.248414973128419No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC10270.24673324700472563No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG10190.24481127429193325No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG10040.24120757545544746No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9930.2385648629753579No Hit
CAGCTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG9840.23640264367346644No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTCGTTTTGTTAG9790.23520141072797118No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT9610.23087697212418826No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT9540.2291952460004949No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT9540.2291952460004949No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT9440.22679278010950438No Hit
GAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCCAAAGA9390.22559154716400914No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC9350.22463056080761296No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9220.22150735514932526No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACGAAAAGATGGGGTTACT9200.22102686197112714No Hit
GTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTC9130.21934513584743381No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA8960.21526094383274996No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA8830.21213773817446227No Hit
GTAAAGAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCC8800.2114169984071651No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA8550.20541083367968885No Hit
ATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8290.19916442236311352No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT8140.19556072352662773No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8120.1950802303484296No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGCTA8060.1936387508138353No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA7870.18907406562095336No Hit
ATGTAAAGAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATAC7710.18523012019536853No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA7710.18523012019536853No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC7690.1847496270171704No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT7570.1818666679479818No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTT7510.1804251884133875No Hit
GGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT7500.18018494182428846No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC7380.1773019827550998No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA7310.17562025663140649No Hit
GTTAGCAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT7270.17465927027501027No Hit
GCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGC7240.1739385305077131No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT7140.1715360646167226No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC7090.17033483167122734No Hit
GGGTATACATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTAC6890.16552989988924632No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG6850.1645689135328501No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6730.16168595446366152No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT6680.16048472151816623No Hit
GGCCTATTGATTGGAAAGTCTGTCAAAGAATTGTGGGTCTTTTGGGTTTT6670.1602444749290672No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC6560.15760176244897764No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA6530.15688102268168047No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC6480.1556797897361852No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6470.15543954314708616No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6390.15351757043429376No Hit
GGTATACATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACA6340.1523163374887985No Hit
GGTTTTGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT6310.15159559772150136No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA6230.14967362500870893No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6170.14823214547411465No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA6150.14775165229591652No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGCTAACAGG6130.14727115911771843No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG6050.14534918640492603No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG6020.14462844663762886No Hit
CTTTCCAATCAATAGGCCTGTTAGCAGGAAGTTTTCTAAAACATTCTTTG6010.1443882000485298No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5980.14366746028123265No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5890.1415052409793412No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5870.1410247478011431No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5870.1410247478011431No Hit
ACCCAAAAGACCCACAATTCTTTGACAGACTTTCCAATCAATAGGCCTGT5820.13982351485564784No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT5800.13934302167744975No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5740.13790154214285544No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5730.13766129555375636No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5710.13718080237555827No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA5670.13621981601916205No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTCGTTTTG5660.13597956943006304No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC5630.13525882966276587No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA5610.13477833648456775No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5600.13453808989546873No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5590.13429784330636965No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5550.13333685694997346No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT5540.1330966103608744No Hit
GCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGG5510.13237587059357725No Hit
TTCTTTGACAGACTTTCCAATCAATAGGCCTGTTAGCAGGAAGTTTTCTA5490.13189537741537916No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA5480.13165513082628008No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC5460.131174637648082No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5420.13021365129168577No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5420.13021365129168577No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5410.12997340470258675No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5400.12973315811348768No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5380.12925266493528959No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC5330.12805143198979432No Hit
TCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTG5300.12733069222249715No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGG5250.12612945927700192No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTA5220.12540871950970475No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAGCAGGAAGTTTTCTAAAAC5190.1246879797424076No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5170.1242074865642095No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTCGT5080.12204526726231804No Hit
GACCTGGCCGTTGCCGTGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5000.12012329454952562No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5000.12012329454952562No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4990.11988304796042656No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA4970.11940255478222847No Hit
ACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCG4950.11892206160403038No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTCGTTT4930.11844156842583228No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT4910.11796107524763416No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC4900.11772082865853513No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4860.11675984230213891No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG4840.11627934912394082No Hit
GTATACATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACAT4820.11579885594574273No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4790.11507811617844557No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA4770.11459762300024745No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT4750.11411712982204936No Hit
GTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGG4720.11339639005475219No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA4690.11267565028745503No Hit
GTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACA4650.11171466393105885No Hit
GGCCTGTTAGCAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT4590.11027318439646454No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC4560.10955244462916738No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG4550.10931219804006832No Hit
CAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTG4540.10907195145096929No Hit
GGATGAAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCA4530.10883170486187023No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT4530.10883170486187023No Hit
CAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4490.10787071850547401No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAT4490.10787071850547401No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC4470.10739022532727592No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA4430.1064292389708797No Hit
GGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTC4390.1054682526144835No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTC4380.10522800602538446No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT4370.10498775943628541No Hit
CTGATGGCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACA4360.10474751284718635No Hit
TTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA4350.10450726625808729No Hit
CCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4340.10426701966898826No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC4310.1035462799016911No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4310.1035462799016911No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4280.10282554013439395No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4250.10210480036709679No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4250.10210480036709679No Hit
CCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTC4250.10210480036709679No Hit
ATTGGGGACCCTGCGCTGAACATGGAGAACATCACATCAGGATTCCTAGG4220.10138406059979964No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG4200.10090356742160154No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC4200.10090356742160154No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG4170.10018282765430439No Hit
GAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGAT4170.10018282765430439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)