FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524739
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTGGTCTGAAAGTAATGATTAACTAGA27420.5225454940456112No Hit
GTGTAAATAGTGTCTGGTCTGAAAGTAATGATTAACTAGATGTTCTGGGT20520.3910515513426675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19260.36703961397952123No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTACTTTTCCTAAC14350.27346928663583225No Hit
CATTTACACCAAGACATCATCAACAAATGTGAACAGTTTGTAGGCCCTCT13070.24907620740977895No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12360.2355456712765775No Hit
GGGTAAAGGTTCATGTATTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGA11740.22373027352645794No Hit
CATTTAAACCCTAACAAGACGAAAAGATGGGGTTATTCTCTACACTTCAT11570.22049056769174769No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11130.21210544670779186No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11040.21039030832471No Hit
GTGTAGAATGTGCTCTTGTGGCAATGATCCCCAACAACCAATGACATACC9060.17265726389690875No Hit
GCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACGATTCTTTGACATACT8990.17132326737673395No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTCAACTTGTGT8980.1711326964452804No Hit
GAATACATGCATACAAGGGCATTAAAGCTGGATAACCACATTGCGTAAAA8850.1686552743363844No Hit
GTGTAGAGAATAACCCCATCTTTTCGTCTTGTTAGGGTTTAAATGTATGC8800.16770241967911667No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCCAAAA8680.16541556850167416No Hit
CATTCTACACAAGTTGAAAGAATGTTTTAGAAAACTCCCTGTTAACAGGC8560.16312871732423168No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8520.16236643359841751No Hit
GTATTAAACCTTACTACCCAGAACATCTAGTTAATCATTACTTTCAGACC8260.1574115893806254No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8240.15703044751771833No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8110.1545530254088223No Hit
GTCCAAGAGTCCTCTTATGTAAGATCTTGGGCAAGATACGGTGGGCGTTC7960.15169446143701917No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTACTTTTC7910.15074160677975146No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7840.14940761025957666No Hit
GCCTAGCAGCCATGGAAATGACGTATATTTGCGGGAGAGAACAACAGAGT7770.14807361373940187No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAACACATAGCGCC7680.14635847535632No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7610.14502447883614522No Hit
ACCCTAACGTTACCAAATATTTACCCTTAGATAAGGGTATTAAACCTTAC7590.1446433369732381No Hit
GTAGAGAATAACCCCATCTTTTCGTCTTGTTAGGGTTTAAATGTATGCCC7550.14388105324742395No Hit
GTCTTTAACCCTCATTGGAAAACACCTACTTTTCCTAACATACATTTACA7550.14388105324742395No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7510.1431187695216098No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATTGCTCA7410.14121306020707436No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCCAAAA7400.14102248927562083No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAG7350.14006963461835312No Hit
TCTCTACACTTCATGGGGTATGTCATTGGTTGTTGGGGATCATTGCCACA7260.13835449623527124No Hit
CATGCATACAAGGGCATTAAAGCTGGATAACCACATTGCGTAAAAGGGGC7060.13454307760620043No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7060.13454307760620043No Hit
GTCTTTGAAGTATTGCTCAAGATCGGTCGTTGACAGTTTGGAGAGTCCAA6970.13282793922311853No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCGACCACC6970.13282793922311853No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGGAGTTTTCTAAAAC6840.1303505171142225No Hit
CCCTTAGATAAGGGTATTAAACCTTACTACCCAGAACATCTAGTTAATCA6840.1303505171142225No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC6770.12901652059404772No Hit
ATTCTACACAAGTTGAAAGAATGTTTTAGAAAACTCCCTGTTAACAGGCC6750.12863537873114061No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6660.12692024034805877No Hit
CCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATTGCTCAA6650.1267296694166052No Hit
CTTTTACGCAATGTGGTTATCCAGCTTTAATGCCCTTGTATGCATGTATT6640.12653909848515166No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG6490.12368053451334855No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6470.12329939265044146No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6430.1225371089246273No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA6400.12196539613026666No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6370.12139368333590604No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACCTCCGGA6330.12063139961009187No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6290.1198691158842777No Hit
GAATAACCCCATCTTTTCGTCTTGTTAGGGTTTAAATGTATGCCCAAAGA6200.11815397750119584No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGACGGT6190.11796340656974229No Hit
ATTCTCTACACTTCATGGGGTATGTCATTGGTTGTTGGGGATCATTGCCA6140.11701055191247459No Hit
ATGGTAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAGCC6090.11605769725520688No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTA6050.1152954135293927No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTTCTGCATGGTCCCGTG6030.11491427166648563No Hit
CCCTAACGTTACCAAATATTTACCCTTAGATAAGGGTATTAAACCTTACT6010.11453312980357853No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATCGTGGGTCTTTTGGGTTTT5970.11377084607776437No Hit
CTCCAAACTGTCAACGACCGATCTTGAGCAATACTTCAAAGACTGTTTGT5950.11338970421485728No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5920.11281799142049667No Hit
CTTCTGCGACGCGGCGATTGAGACCGTCGTCTGCGAGGCGAGGGAGTTCT5880.11205570769468248No Hit
GGTTCATGTATTGTTTACAGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5820.11091228210596125No Hit
GTATGCATGTATTCAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACA5800.11053114024305417No Hit
GTCTAGAAGATCCCGTACAGACGGAAAGAAATCAGAAGGCAAAAATGAGA5800.11053114024305417No Hit
GCTCAAGATCGGTCGTTGACAGTTTGGAGAGTCCAAGAGTCCTCTTATGT5740.10938771465433292No Hit
GTTAACAGGGAGTTTTCTAAAACATTCTTTCAACTTGTGTAGAATGTGCT5650.10767257627125106No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5630.10729143440834396No Hit
GCACGGGACCATGCAGAACCTGCATGACTACTGCTCAAGGAACCTCTATG5580.10633857975107626No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG5560.10595743788816916No Hit
CTATTTACACACTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAA5530.10538572509380854No Hit
CTCTTACTGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGG5510.10500458323090145No Hit
CTTTAACCCTCATTGGAAAACACCTACTTTTCCTAACATACATTTACACC5490.10462344136799437No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5470.1042422995050873No Hit
ACCAATGACATACCCCATGAAGTGTAGAGAATAACCCCATCTTTTCGTCT5470.1042422995050873No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC5410.10309887391636605No Hit
ATATTTACCCTTAGATAAGGGTATTAAACCTTACTACCCAGAACATCTAG5400.10290830298491249No Hit
CTCTTGGACTCTCCAAACTGTCAACGACCGATCTTGAGCAATACTTCAAA5360.10214601925909834No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5340.10176487739619125No Hit
GACATACCCCATGAAGTGTAGAGAATAACCCCATCTTTTCGTCTTGTTAG5310.10119316460183063No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5270.10043088087601645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)