FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149328
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14720.9857494910532518No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA12550.8404318011357548No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9460.6335047680274295No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCGCGCAGCCGGTCTGGTGC8550.5725650916104146No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7180.48082074359798566No Hit
GTTTCGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6510.43595306975249115No Hit
ACTTATCGGGACTGACAATTCTGTCGTACTATCCCGCAAATATACATCGT5530.3703257259187828No Hit
GCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGG4960.33215471981142186No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4310.2886263795135541No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTTTGGA4210.28192971177542053No Hit
GCTCAGAATACTGCCTCTGCCATATCATCAACCTTCTCGAAGACTGGGGA4170.27925104468016715No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT3990.26719704275152684No Hit
CGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCAT3810.2551430408228865No Hit
GTACAGATTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA3790.2538037072752598No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3620.24241937212043288No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3560.23840137147755278No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3530.23639237115611272No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT3380.22634736954891244No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA3340.22366870245365905No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3310.221659702132219No Hit
AAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGT3210.2149630343940855No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3200.21429336762027215No Hit
GGTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGT3150.2109450337512054No Hit
CAAATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTG3140.21027536697739205No Hit
CCCCAACTCCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCT2980.19956069859637846No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG2880.19286403085824494No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA2810.18817636344155148No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA2810.18817636344155148No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2790.18683702989392478No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT2740.18348869602485804No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC2670.17880102860816457No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC2660.17813136183435124No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAGGAGACTA2650.17746169506053788No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC2640.17679202828672452No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT2620.17545269473909783No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTCTGGGGTTC2570.17210436087003106No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG2510.16808636022715095No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC2410.16138969248901747No Hit
CCTCTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGT2250.15067502410800385No Hit
GGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG2240.15000535733419051No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTGTGT2240.15000535733419051No Hit
GGAACATATTGTACACAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA2230.14933569056037715No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAAT2220.14866602378656382No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT2220.14866602378656382No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG2200.1473266902389371No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2200.1473266902389371No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC2160.14464802314368372No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA2130.14263902282224364No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT2100.1406300225008036No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA2100.1406300225008036No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG2100.1406300225008036No Hit
ATGTTTCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGCACCGG2100.1406300225008036No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA2080.1392906889531769No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA2080.1392906889531769No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG2070.13862102217936353No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGTACGACAGAAT2060.1379513554055502No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA2040.1366120218579235No Hit
CAGTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG2030.13594235508411015No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG2020.1352726883102968No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG2010.13460302153648346No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA2010.13460302153648346No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA1970.13192435444123005No Hit
GAATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGGCTCTACCGCCCGC1970.13192435444123005No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC1950.13058502089360335No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT1950.13058502089360335No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC1940.12991535411979No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA1920.12857602057216327No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTTTCCATGC1920.12857602057216327No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC1920.12857602057216327No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG1910.12790635379834994No Hit
CCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA1890.12656702025072322No Hit
CATATTGTACACAAAATCAAATTATGTTTTAGGAAACTTCCTGTAAACAG1870.12522768670309653No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT1870.12522768670309653No Hit
CTATTATCCCGAGCATGTGGTTAATCATTACTTCCAGACGAGACATTATT1840.12321868638165648No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG1840.12321868638165648No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT1840.12321868638165648No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC1840.12321868638165648No Hit
ATGTATATTTGCGGGATAGTACGACAGAATTGTCAGTCCCGATAAGTTTT1830.12254901960784313No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCG1830.12254901960784313No Hit
ATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGGCTCTACCGCCCGCTC1830.12254901960784313No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA1830.12254901960784313No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG1820.12187935283402979No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA1820.12187935283402979No Hit
GAACAAACGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC1810.12120968606021644No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTGTGTACAATATGTT1810.12120968606021644No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1800.1205400192864031No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG1780.11920068573877639No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG1760.11786135219114968No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG1760.11786135219114968No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA1750.11719168541733634No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC1750.11719168541733634No Hit
CCACCGCACGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAG1730.11585235186970964No Hit
ATATCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAGACTC1730.11585235186970964No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG1710.11451301832208294No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT1710.11451301832208294No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT1700.11384335154826959No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTGTGTC1680.11250401800064287No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA1670.11183435122682953No Hit
GCTTGTTTTGCGCGCAGCCGGTCTGGTGCAAAACTTATCGGGACTGACAA1670.11183435122682953No Hit
GGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTG1640.10982535090538947No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG1630.10915568413157613No Hit
CATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTGC1630.10915568413157613No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT1620.10848601735776277No Hit
GAATATCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAGAC1600.10714668381013608No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG1590.10647701703632273No Hit
GTCCTTTGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCC1590.10647701703632273No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC1580.10580735026250937No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG1580.10580735026250937No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC1570.10513768348869602No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG1540.10312868316725597No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCC1540.10312868316725597No Hit
ATGTAATTGGGAGTTGGGGTTCCTTGCCACAGGAACATATTGTACACAAA1540.10312868316725597No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAACATCTTGAGTCCCTTTATGCC1540.10312868316725597No Hit
AATCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT1530.10245901639344263No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG1520.10178934961962928No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTA1500.10045001607200257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)