FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306999
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA31641.0306222495838748No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA27360.8912081146844126No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17430.5677542923592585No Hit
TCTTTGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTC16340.532249290714302No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA15500.5048876380704823No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG14370.468079700585344No Hit
AGCCCAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTT11850.3859947426538849No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11440.3726396502920205No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT10090.32866556568588173No Hit
CTGTCTCGGGAGGCCTTAGAGTCTCCAGAACATTGTTCACCTCACCATAC9230.3006524451219711No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9010.2934862980009707No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8830.2876230867201522No Hit
AAGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATACTCAAGA8820.2872973527601067No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8770.28566868295987935No Hit
ACACTTGGGAGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA8430.2745937283183333No Hit
GTGGCCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7840.25537542467565044No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC7450.242671800233877No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG7130.2322483135124219No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6970.2270365701516943No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC6410.20879546838914786No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGTCT6070.19772051374760177No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6050.19706904582751086No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5720.18631982514601025No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCACG5640.18371395346564648No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT5470.17817647614487342No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5460.17785074218482796No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG5450.1775250082247825No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5420.17654780634464606No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAA5170.16840445734350926No Hit
TCTTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTC5140.16742725546337284No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG5070.16514711774305454No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5050.16449564982296358No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4970.1618897781425998No Hit
CCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATCGAAGGAAAG4960.16156404418255435No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4850.15798097062205413No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4760.1550493649816449No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTTCACCTCACCATACAG4730.15407216310150848No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATACTCAAGAA4720.153746429141463No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAG4610.1501633555809628No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTG4600.14983762162091732No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4590.14951188766087187No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT4460.14527734618028074No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG4440.14462587826018977No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4380.14267147449991693No Hit
TTCTAGGCTTTGCCGCTCCCTTTACACAATGTGGTTACCCAGCATTAATG4370.14234574053987148No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTGTAAA4170.135831061338962No Hit
GCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4130.13452812549878013No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4110.1338766575786892No Hit
CTACTTTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT4090.13322518965859823No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA4080.13289945569855277No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4070.1325737217385073No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4030.13127078589832541No Hit
GTATAGGCCCCTCACTCTGGGATCTAGTAGAGCTTGGTGGAATGTGCTGG3970.12931638213805255No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACT3960.1289906481780071No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3920.12768771233782522No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3900.12703624441773426No Hit
GCCTTAGAGTCTCCAGAACATTGTTCACCTCACCATACAGCACTAAGGCA3850.1254075746175069No Hit
ACACGTGGGAGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA3840.12508184065746142No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3760.12247596897709764No Hit
GCCCAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT3750.12215023501705216No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG3750.12215023501705216No Hit
AGCCAAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTT3700.12052156521682482No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTACTAG3670.11954436333668839No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGTTGAGTTGATGAATCTGGCCAC3620.11791569353646104No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG3620.11791569353646104No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT3570.11628702373623367No Hit
ACTCACCCCAACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAA3550.11563555581614272No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3530.11498408789605177No Hit
CATTAAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGATTGAGACCT3520.11465835393600629No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC3500.11400688601591537No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG3500.11400688601591537No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG3480.11335541809582442No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3430.11172674829559705No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3380.11009807849536969No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT3370.10977234453532422No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACT3300.1074922068150059No Hit
GTGTTGGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3300.1074922068150059No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3290.10716647285496045No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3260.10618927097482403No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCCTGCATGGTCCCGTG3260.10618927097482403No Hit
GGAGACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAGA3260.10618927097482403No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT3260.10618927097482403No Hit
TTTACAGACTGGGAGGAGTTGGGGGAGGAGATTAGAC3210.10456060117459666No Hit
AGCCCAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTA3210.10456060117459666No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA3170.10325766533441477No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3160.1029319313743693No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATAC3140.10228046345427835No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGT3140.10228046345427835No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA3110.10130326157414193No Hit
GGTCCTGCATGGTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGAC3100.10097752761409645No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3090.10065179365405098No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3070.10000032573396006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)