FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306999
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA27690.9019573353659133No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA26550.8648236639207293No Hit
TCTTTGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTC16990.5534219981172577No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15770.5136824549917101No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG13050.4250828178593416No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA12960.4221512122189323No Hit
AGCCCAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTT10170.3312714373662455No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10150.33061996944615457No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT9900.32247662044501774No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8800.28664588484001574No Hit
CTGTCTCGGGAGGCCTTAGAGTCTCCAGAACATTGTTCACCTCACCATAC8630.28110840751924276No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8540.2781768018788335No Hit
AAGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATACTCAAGA8340.271662122677924No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8190.266776113277242No Hit
ACACTTGGGAGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7720.2514666171551047No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGT7570.24658060775442264No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC7000.22801377203183074No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6710.218567487190512No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6610.2153101475900573No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC6400.20846973442910235No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6350.206841064628875No Hit
GTGGCCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6170.2009778533480565No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT5910.19250877038687422No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5610.18273675158551006No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5570.18143381574532816No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCACG5510.17947941198505532No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG5510.17947941198505532No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5460.17785074218482796No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAG5310.17296473278414587No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGTCT5290.17231326486405493No Hit
TCTTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTC5060.16482138378300906No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG4930.1605868423024179No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4850.15798097062205413No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT4810.15667803478187226No Hit
TTCTAGGCTTTGCCGCTCCCTTTACACAATGTGGTTACCCAGCATTAATG4660.15179202538119016No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTTCACCTCACCATACAG4560.14853468578073545No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG4540.1478832178606445No Hit
CTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG4520.14723174994055355No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAA4510.14690601598050806No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4450.14495161222023525No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4420.14397441034009884No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATACTCAAGAA4310.14039133677959864No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4300.14006560281955316No Hit
CCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATCGAAGGAAAG4220.13745973113918938No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA4200.13680826321909842No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4130.13452812549878013No Hit
TTTACAGACTGGGAGGAGTTGGGGGAGGAGATTAGAC4130.13452812549878013No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4040.13159651985837087No Hit
GCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4010.13061931797823445No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3970.12931638213805255No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC3910.12736197837777974No Hit
GTATAGGCCCCTCACTCTGGGATCTAGTAGAGCTTGGTGGAATGTGCTGG3840.12508184065746142No Hit
CTACTTTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT3810.12410463877732501No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT3780.1231274368971886No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3760.12247596897709764No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACT3690.12019583125677934No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT3610.11758995957641556No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA3560.11596128977618819No Hit
GCCTTAGAGTCTCCAGAACATTGTTCACCTCACCATACAGCACTAAGGCA3550.11563555581614272No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG3540.11530982185609724No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3540.11530982185609724No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG3500.11400688601591537No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA3500.11400688601591537No Hit
ACTCACCCCAACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAA3490.1136811520558699No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG3480.11335541809582442No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTACTAG3410.1110752803755061No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTTTGCCAAGTGTTTGCTG3400.11074954641546064No Hit
GGAGACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAGA3400.11074954641546064No Hit
ACACGTGGGAGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA3370.10977234453532422No Hit
CGGTGGCCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3370.10977234453532422No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG3350.10912087661523327No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3340.1087951426551878No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3310.10781794077505137No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3280.10684073889491498No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTGTAAA3250.10586353701477855No Hit
AGCCAAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTT3240.10553780305473308No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3220.10488633513464213No Hit
CATTAAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGATTGAGACCT3220.10488633513464213No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3200.10423486721455118No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC3190.10390913325450571No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT3170.10325766533441477No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGTTGAGTTGATGAATCTGGCCAC3120.1016289955341874No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3120.1016289955341874No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC3100.10097752761409645No Hit
CTACTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT3090.10065179365405098No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3080.1003260596940055No Hit
GCCCAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT3080.1003260596940055No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3080.1003260596940055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)