FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509800
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA24380.47822675559042765No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19710.38662220478619064No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC19580.38407218517065517No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15680.30757159670459006No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG15200.2981561396626128No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14300.28050215770890546No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC13440.26363279717536287No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12480.24480188309140838No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC12430.24382110631620244No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA12090.23715182424480186No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12050.23636720282463713No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG12010.23558258140447233No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11480.22518634758728912No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA11120.2181247548058062No Hit
CCCTTAACTTCATGGGATATGTAATCGGAAGTTGGGGTACCTTGCCACAG11110.21792859945076504No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT10850.212828560219694No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG10650.20890545311887015No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC10390.20380541388779913No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCGATTACATATCCCATGAA10380.20360925853275796No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC10040.1969399764613574No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG9530.18693605335425656No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG9490.18615143193409178No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9440.18517065515888584No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9410.18458218909376226No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG9300.18242448018830915No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9250.18144370341310317No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9130.17908983915260887No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG9080.1781090623774029No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9070.1779129070223617No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9070.1779129070223617No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8790.17242055708120832No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8600.16869360533542566No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT8550.1677128285602197No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC8540.1675166732051785No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8460.16594743036484896No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG8330.16339741074931344No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8110.15908199293840722No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7760.15221655551196547No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7700.15103962338171834No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7620.14947038054138878No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTG7580.148685759121224No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7500.14711651628089448No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC7490.14692036092585328No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7490.14692036092585328No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT7420.14554727344056492No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7350.1441741859552766No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7330.1437818752451942No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGAAAACTTCCTATAA7270.14260494311494704No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA7240.14201647704982348No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT7220.14162416633974106No Hit
ATTTATAGGAAGTTTTCGAAAACATTGTTTCAATTTTTGTACAATATGTT7200.1412318556296587No Hit
CCCCAAAAGACTGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGATCCCA7160.1404472342094939No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7080.13887799136916437No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATATT7030.13789721459395843No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACACGCAGTGCC7020.1377010592389172No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6970.13672028246371126No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTG6940.13613181639858768No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6940.13613181639858768No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC6910.1355433503334641No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6890.13515103962338174No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA6740.13220870929776385No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGG6720.13181639858768143No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6700.13142408787759904No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6640.1302471557473519No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6610.12965868968222832No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6540.12828560219694No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6430.12612789329148685No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA6420.12593173793644566No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6400.12553942722636327No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6380.12514711651628088No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6370.1249509611612397No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6360.12475480580619852No Hit
TTATTATCCTGAACACACAGTTAATCATTACTTCCAAACTAGACATTATT6320.12397018438603374No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACACGC6310.12377402903099254No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6230.12220478619066301No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6210.12181247548058063No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG6190.12142016477049823No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6180.12122400941545705No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCGGTAAAGTTTCCCAC6180.12122400941545705No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6160.12083169870537465No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6100.1196547665751275No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACAC6070.11906630051000393No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6050.11867398979992154No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6040.11847783444488034No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6030.11828167908983914No Hit
ACTACACGCAGTGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA5980.11730090231463319No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5980.11730090231463319No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5980.11730090231463319No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5940.11651628089446842No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5940.11651628089446842No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACACGCAGTGCCTCAT5920.11612397018438603No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5910.11592781482934483No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5880.11533934876422126No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5870.11514319340918007No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5860.11494703805413889No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5860.11494703805413889No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACCTTT5830.1143585719890153No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5810.1139662612789329No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5800.11377010592389172No Hit
GGGTGTACTGTTCCGGAACTTGAGCCACCAGCAGGAAAGTATAGGCCCCT5780.11337779521380933No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5780.11337779521380933No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5730.11239701843860338No Hit
CAGTTAATCATTACTTCCAAACTAGACATTATTTACATACTCTGTGGAAG5710.11200470772852097No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5710.11200470772852097No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG5640.11063162024323263No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5600.10984699882306787No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5550.10886622204786191No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5530.10847391133777952No Hit
GGTCTATTTATAGGAAGTTTTCGAAAACATTGTTTCAATTTTTGTACAAT5510.10808160062769714No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTGTGTTCA5480.10749313456257356No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5470.10729697920753237No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTC5460.10710082385249117No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5420.1063162024323264No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5410.10612004707728522No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5340.10474695959199687No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5340.10474695959199687No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC5340.10474695959199687No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5330.10455080423695567No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5310.10415849352687329No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5310.10415849352687329No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGACCTTTAATCTAATCTCCTCC5300.10396233817183209No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC5280.1035700274617497No Hit
GTACAGTAGAAGAATAAAGCCCGGTAAAGTTTCCCACCTTATGAGTCCAA5280.1035700274617497No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC5230.10258925068654375No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC5210.10219693997646136No Hit
GTTAATCATTACTTCCAAACTAGACATTATTTACATACTCTGTGGAAGGC5210.10219693997646136No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT5180.10160847391133777No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTGTACTGTTCCGGAACTTGAG5170.10141231855629658No Hit
CCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATG5160.1012161632012554No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5160.1012161632012554No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCAGCACATT5120.10043154178109064No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGATTCAAGATG5100.10003923107100823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)