FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327594
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGGACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC38361.1709616171236348No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC25530.7793183025330134No Hit
GCCTAGTCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGT24960.7619187164600084No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA24720.7545925749555853No Hit
CATTCTGTGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGG13420.40965341245566156No Hit
GTACACCACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC13090.3995799678870798No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC12490.38126461412602186No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT11590.353791583484435No Hit
GCCTAGTCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGT11540.3522653040043468No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10510.3208239467145308No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC10230.3122767816260371No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT9480.28938258942471473No Hit
CCCGTAAAATGAGGCGCTACGTGTGGACTCTCTTTTATATAAGATCCCCG9180.2802249125441858No Hit
ATTCTGTGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGT9040.27595132999993893No Hit
CAGCCGGTCTGGGGCGAAACTCATCGGGACTGACAACTCTGTCGTGCTCT8940.2728987710397626No Hit
ATTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGT8790.26831993259949816No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8550.260993791095075No Hit
CCCACAGAATGAGGCGCTACGTGTGGACTCTCTTTTATATAAGATCCCCG8430.2573307203428634No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8290.2530571377986166No Hit
ATATACATGTATACAAGCAAAACAGGCCTTTACTTTCTCGCCAACTTACA8280.25275188190259895No Hit
GTGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG7890.2408469019579113No Hit
ACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTT7780.2374890871017174No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7730.23596280762162922No Hit
GTACCGAACATGGAGAACATCGCATCCGGACTCCTAGGACCCCTGCTCGT7640.23321550455747053No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7450.22741564253313554No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC7030.21459489490039502No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6740.20574247391588368No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6720.2051319621238484No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6440.19658479703535472No Hit
CTAGTCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCT6340.19353223807517841No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAGT6280.19170070269907263No Hit
CATGTATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAAGGGGC6070.18529032888270236No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA6040.18437456119464948No Hit
CTTTAGGACATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA5890.17979572275438502No Hit
GTCCTACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5890.17979572275438502No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5860.1788799550663321No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5850.17857469917031446No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5850.17857469917031446No Hit
CATGTATACAAGCAAAACAGGCCTTTACTTTCTCGCCAACTTACAAGGCT5820.17765893148226158No Hit
TCTTTCTGTCCCCAATCCCCTGGGATTCTTTCCCGATCATCAGTTGGACC5790.17674316379420868No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5690.17369060483403237No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5600.17094330176987368No Hit
CTTACAGGTGGAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGT5580.17033278997783843No Hit
CTTTAGGACATGGACATTGACACGTATAAAGAATTTGGAAATGTCAACGA5570.17002753408182078No Hit
GTTCTGTACAAGGTCCCCAATCTTCGATAAGATTGACGATATGGCAGAGA5550.16941702228978553No Hit
GGTGTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5470.1669749751216445No Hit
CTGTAAGGGGCCCCACAAATTGCGTACATCTATCAACAATGTCCTCCTTC5390.16453292795350344No Hit
GAGCCTGAGGGCTCCACCCCAACAGTCCCCCATGGGGAGGGGTGAACCCT5360.16361716026545053No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5350.16331190436943288No Hit
AGTCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGG5210.15903832182518604No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG5100.1556805069689921No Hit
GGGCAAAACTCATCGGGACTGACAACTCTGTCGTGCTCTCCCGCAAGTAT5040.15384897159288632No Hit
GTATACATGTATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAA4870.14865962136058658No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGAGGGCGTTC4850.1480491095685513No Hit
ATCCAGCATCCAGGGAAGCAGTAGTCAGCTATGTCAACATTAATATGGGC4850.1480491095685513No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4720.1440807829203221No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG4700.14347027112828684No Hit
GGGGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCT4590.1401124562720929No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAGTTGTCAGTCCCGATG4530.13828092089598712No Hit
CCTTTATATACATGTATACAAGCAAAACAGGCCTTTACTTTCTCGCCAAC4500.13736515320793422No Hit
ATCTTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT4480.13675464141589896No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGAGGGCGTTCACGGTGGTCTC4470.1364493855198813No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4430.13522836193581078No Hit
ACCTTGTACCGAACATGGAGAACATCGCATCCGGACTCCTAGGACCCCTG4280.13064952349554632No Hit
ATGCAAGACCTTGGGCAGGTTCCCGAGGGCGTTCACGGTGGTCTCCATGC4250.12973375580749344No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG4220.1288179881194405No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGAAAC4200.12820747632740526No Hit
ACCTTGGGCAGGTTCCCGAGGGCGTTCACGGTGGTCTCCATGCGACGTGC4110.12546017326324657No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4080.12454440557519368No Hit
CAGCCGGTCTGGGGCAAAACTCATCGGGACTGACAACTCTGTCGTGCTCT4080.12454440557519368No Hit
CCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA4060.12393389378315843No Hit
CCTCCTCCATATAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGA4050.12362863788714079No Hit
ATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAA4040.12332338199112317No Hit
GTGTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGT4030.12301812609510554No Hit
GGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT3970.12118659071899973No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3960.1208813348229821No Hit
GCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGT3950.12057607892696448No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3950.12057607892696448No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGAAACAGTGAGCCCTGCTCAGA3920.11966031123891159No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA3880.11843928765484105No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC3870.11813403175882342No Hit
ATCTTTCTGTCCCCAATCCCCTGGGATTCTTTCCCGATCATCAGTTGGAC3780.11538672869466475No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3760.11477621690262949No Hit
TCCAAACGCGACATTATTTACACACTCTTTGGAAGGCGGGGATCTTATAT3750.11447096100661183No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCTCGGGAACCTGCCC3740.1141657051105942No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG3740.1141657051105942No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG3700.11294468152652369No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3690.11263942563050605No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3620.11050263435838263No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG3620.11050263435838263No Hit
GTAGAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG3590.10958686667032974No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA3540.10806058719024159No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT3510.1071448195021887No Hit
ATGTGGATATCCTGCTTTGATGCCTTTATATACATGTATACAAGCAAAAC3490.10653430771015342No Hit
GGTGTCTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG3480.10622905181413579No Hit
GATGTATACTTGCGGGAGAGCACGACAGAGTTGTCAGTCCCGATGAGTTT3430.10470277233404764No Hit
GAGTCGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCT3420.10439751643803001No Hit
GGCCTTTACTTTCTCGCCAACTTACAAGGCTTTTCTGAAAAACCAGTATC3410.10409226054201237No Hit
CTAGTCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCT3380.10317649285395947No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3340.10195546926988895No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3320.10134495747785369No Hit
TCCTTCCCCTGGCTCTAACAACAGCAGTTTCCGGAAGTGTTGACAAGATA3320.10134495747785369No Hit
ATATTAATGTTGACATAGCTGACTACTGCTTCCCTGGATGCTGGATCGTC3290.1004291897898008No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3290.1004291897898008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)