FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454454
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACGAGCTGTCAGATTTTTTAATAGGGAGGATGGGGTTGTTATATGGA15780.34722986264836486No Hit
GTATAGGGGTTAGGGACTACAGGATGAATAGGGAGGACAGCCTGATTGAT15520.3415087115527644No Hit
GTGCTGGTGGGGATGAGAGGGAGAAGTGTATAGGGGTTAGGGACTACAGG15180.3340272062739023No Hit
GTTATATGGAGTGTTGGTCTAAGACGACTGTGTGAGCAGAGCTTATGATG14640.322144815536886No Hit
GCCTAATCATGCCTCATTGCCCCACCCCAATCCTAGGCCAAGACATTTTA14060.30938224770823886No Hit
TTCCATAGCTGCTCACCAGGACCTCATCAAAATCCAGTTAAAAGACCCCT12370.2721947655868361No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA10960.2411685231068491No Hit
GTGCTGGTGGGAGTGTAGCAGTGGGGACGACCAGAGATCCCTCTCATGGC10290.2264255568220326No Hit
GTGTATAGGGGTTAGGGACTACAGGATGAATAGGGAGGACAGCCTGATTG9950.21894405154317045No Hit
CTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC9780.21520329890373943No Hit
CACCAGGACCTCATCAAAATCCAGTTAAAAGACCCCTCTAAATTTCCCAA9540.20992223635395443No Hit
CTGTAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCATCTATTCGA8760.1927587830671531No Hit
GTATGTAAGTAATGCCTAGTTTTGAAGTAATGATTAACTGCATGGTTAGG8640.1901182517922606No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8080.1777957725094289No Hit
ACCCAGTAGTGTGGGACACCATCACCCCTTCCATAGCTGCTCACCAGGAC7880.1733948870512747No Hit
CCATCACCCCTTCCATAGCTGCTCACCAGGACCTCATCAAAATCCAGTTA7770.1709744000492899No Hit
GTATTAAGAGGTGAGGCCTTTAAGAGGTGATCAGGGCAGGGCACGGTGGC7760.1707543557763822No Hit
TACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACATGAGGGAAAC7550.16613342604532033No Hit
GCCTAGTCATGCCTCATTGCCCCACCCCAATCCTAGGCCAAGACATTTTA7530.1656933374995049No Hit
TGCAATGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTG7490.16481316040787408No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA7480.16459311613496636No Hit
CTGTTGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCATCTATTCGA7400.16283276195170468No Hit
GCCAAGGTCAAATCAGGTAGGAGTGGGAGCGTTCGGGCCAGGATTCACCC7390.16261271767879698No Hit
CCAGGACCTCATCAAAATCCAGTTAAAAGACCCCTCTAAATTTCCCAACA6820.15007019412305755No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6740.14830983993979588No Hit
GCTTTATATAGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCATCA6620.14566930866490338No Hit
GGTATGAGCTGTCAGATTTTTTAATAGGGAGGATGGGGTTGTTATATGGA6390.14060829038802608No Hit
GTAATGTACCCCAACTTCCAATTACATAGCCCATGAAGTTAAGGGAGTAG6370.14016820184221065No Hit
CCCCAGCACCCGCTACCCGCCTCCCTCATTAACCCAGTAGTGTGGGACAC5960.13114638665299458No Hit
GTAAAAAAAGGTTTGTCAGTTTTCCATTATAAAATGTTTGAACTCTCTAA5950.13092634238008688No Hit
CTGCACGGCCTGAGGATGGGTGTCTCTTAGAGGTGGAGAGATGGGAGTAG5920.13026620956136375No Hit
TCCACTGTATGGCCTGAGGATGGGTGTCTCTTAGAGGTGGAGAGATGGGA5910.13004616528845603No Hit
ATATAAAGCTGTGGCGGTGTCGAGGAGATCTCGAATAGATGGAAAGAAGT5810.12784572255937895No Hit
GTTGTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTTCCGTG5720.12586532410320958No Hit
CATTAACCCAGTAGTGTGGGACACCATCACCCCTTCCATAGCTGCTCACC5720.12586532410320958No Hit
GTACCTTACCACAAGATCATATTGTAGAAAAAATCAAGCAATGTTTTCGA5650.12432501419285559No Hit
CAGTAGAAGAATAAAGTCCAGTAAAGTTGCCCACCTTATGAGTCCAAGGG5600.12322479282831705No Hit
ATCATAAGCTCTGCTCACACAGTCGTCTTAGACCAACACTCCATATAACA5560.12234461573668622No Hit
GCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGG5490.12080430582633225No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCGTG5260.11574328754945495No Hit
GTTATTAGACGACGAGGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCG5240.11530319900363954No Hit
GGCCATACAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCA5210.11464306618491639No Hit
GCCACTGGCCAATGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT5210.11464306618491639No Hit
ATATAAAAGAGAAACTACACGAAGCGCCTCATTTTGTGGGTCACCATATT5100.1122225791829316No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG4980.1095820479080391No Hit
CCATATAACAACCCCATCCTCCCTATTAAAAAATCTGACAGCTCATACCA4910.10804173799768514No Hit
GTAGTGTGGGACACCATCACCCCTTCCATAGCTGCTCACCAGGACCTCAT4890.10760164945186972No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4850.10672147236023889No Hit
CCATATAACAACCCCATCCTCCCTATTAAAAAATCTGACAGCTCGTACCA4700.10342080826662324No Hit
CCCTAACACACCAAAGGACTTACAACCCATCATAAGCTCTGCTCACACAG4650.10232058690208469No Hit
GTGCTGGTGTTTGTGTGTATAATTGAATATTATTCAGCTTTTGTAAAGAA4620.10166045408336156No Hit
GTGCTGGTGGGAGAGTAGCAGTGAGGACGACCAGAGTCACTCTCATCGCA4610.10144040981045387No Hit
GTATACATGCGTACAAAGGCATTAAGGCTGGATACCCACATTGTGTAAAA4560.10034018844591532No Hit
ATCTGACCCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4550.1001201441730076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)