FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454454
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGGGACTACAGGATGAATAGGGAGGACAGCCTGATTGAT16320.35911225338538116No Hit
GGTACGAGCTGTCAGATTTTTTAATAGGGAGGATGGGGTTGTTATATGGA15310.33688778182170254No Hit
GTTATATGGAGTGTTGGTCTAAGACGACTGTGTGAGCAGAGCTTATGATG15200.33446729481971776No Hit
GTGCTGGTGGGGATGAGAGGGAGAAGTGTATAGGGGTTAGGGACTACAGG14930.3285260994512096No Hit
GCCTAATCATGCCTCATTGCCCCACCCCAATCCTAGGCCAAGACATTTTA12880.2834170235051292No Hit
TTCCATAGCTGCTCACCAGGACCTCATCAAAATCCAGTTAAAAGACCCCT12360.27197472131392836No Hit
GTGTATAGGGGTTAGGGACTACAGGATGAATAGGGAGGACAGCCTGATTG10710.23566741628415636No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA10370.22818591100529426No Hit
GTGCTGGTGGGAGTGTAGCAGTGGGGACGACCAGAGATCCCTCTCATGGC9480.20860197071650816No Hit
CTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC9430.20750174935196963No Hit
GTATGTAAGTAATGCCTAGTTTTGAAGTAATGATTAACTGCATGGTTAGG8670.19077838461098373No Hit
CACCAGGACCTCATCAAAATCCAGTTAAAAGACCCCTCTAAATTTCCCAA8480.18659754342573726No Hit
CTGTAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCATCTATTCGA8280.18219665796758308No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7790.17141448859510533No Hit
ACCCAGTAGTGTGGGACACCATCACCCCTTCCATAGCTGCTCACCAGGAC7530.1656933374995049No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA7030.15469112385411943No Hit
CTGTTGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCATCTATTCGA7030.15469112385411943No Hit
GTATTAAGAGGTGAGGCCTTTAAGAGGTGATCAGGGCAGGGCACGGTGGC6990.1538109467624886No Hit
CCATCACCCCTTCCATAGCTGCTCACCAGGACCTCATCAAAATCCAGTTA6900.15183054830631923No Hit
TGCAATGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTG6580.14478913157327253No Hit
GCCTAGTCATGCCTCATTGCCCCACCCCAATCCTAGGCCAAGACATTTTA6520.14346886593582628No Hit
GGTATGAGCTGTCAGATTTTTTAATAGGGAGGATGGGGTTGTTATATGGA6480.14258868884419545No Hit
GTAAAAAAAGGTTTGTCAGTTTTCCATTATAAAATGTTTGAACTCTCTAA6470.14236864457128773No Hit
GCCAAGGTCAAATCAGGTAGGAGTGGGAGCGTTCGGGCCAGGATTCACCC6340.13950806902348753No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6290.13840784765894898No Hit
GCTTTATATAGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCATCA6290.13840784765894898No Hit
CCAGGACCTCATCAAAATCCAGTTAAAAGACCCCTCTAAATTTCCCAACA6260.13774771484022585No Hit
TACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACATGAGGGAAAC6250.13752767056731816No Hit
GTACCTTACCACAAGATCATATTGTAGAAAAAATCAAGCAATGTTTTCGA6200.1364274492027796No Hit
CTGCACGGCCTGAGGATGGGTGTCTCTTAGAGGTGGAGAGATGGGAGTAG6160.13554727211114878No Hit
GTAATGTACCCCAACTTCCAATTACATAGCCCATGAAGTTAAGGGAGTAG5870.1291659881968252No Hit
CCCCAGCACCCGCTACCCGCCTCCCTCATTAACCCAGTAGTGTGGGACAC5770.12696554546774813No Hit
CATTAACCCAGTAGTGTGGGACACCATCACCCCTTCCATAGCTGCTCACC5720.12586532410320958No Hit
GTTATTAGACGACGAGGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCG5520.12146443864505538No Hit
TCCACTGTATGGCCTGAGGATGGGTGTCTCTTAGAGGTGGAGAGATGGGA5520.12146443864505538No Hit
GGCCATACAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCA5270.11596333182236267No Hit
GTATACATGCGTACAAAGGCATTAAGGCTGGATACCCACATTGTGTAAAA5250.11552324327654725No Hit
GCCACTGGCCAATGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT5150.11332280054747014No Hit
GTTGTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTTCCGTG5120.11266266772874702No Hit
GCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGG4940.10870187081640827No Hit
GTAGTGTGGGACACCATCACCCCTTCCATAGCTGCTCACCAGGACCTCAT4910.10804173799768514No Hit
CAGTAGAAGAATAAAGTCCAGTAAAGTTGCCCACCTTATGAGTCCAAGGG4900.10782169372477743No Hit
ATCATAAGCTCTGCTCACACAGTCGTCTTAGACCAACACTCCATATAACA4900.10782169372477743No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG4860.10694151663314659No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCGTG4800.10562125099570034No Hit
CCATATAACAACCCCATCCTCCCTATTAAAAAATCTGACAGCTCATACCA4680.10298071972080781No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4670.10276067544790012No Hit
CTCCCAGCTTCAGGCGATCCACCTGCCTCAGTCTCCCAAAGTGCTAGGAT4560.10034018844591532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)