FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448904
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC28170.6275283802327446No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA25580.5698323026749594No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA20910.4658011512483738No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG14120.3145438668401262No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA13300.29627715502646446No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12090.26932261686240266No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG12010.26754049863667956No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11740.26152584962486414No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11010.24526402081514087No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10880.24236807869834087No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10840.2414770195854793No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT10810.24080872525083313No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10490.23368025234794076No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10300.2294477215618484No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10060.22410136688467913No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9990.22254201343717145No Hit
GGAATATGATAAAACGCCGCAGATACATCCAGCGATAACCAGGACAAATT9960.22187371910252526No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9710.2163045996471406No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTTGTAACATT9700.2160818348689252No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9660.21519077575606363No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8990.20026553561563273No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT8760.19514194571667884No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8670.19313706271274036No Hit
ACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTCCATGCGACGTGC7870.17531588045550942No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7860.17509311567729405No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7760.17286546789514018No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA7620.16974676100012476No Hit
ATGCCGGATCTTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAAC7530.16774187799618626No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7450.16595975977046318No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA7450.16595975977046318No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7400.16484593587938623No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7300.16261828809723236No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA7090.15794022775470926No Hit
GTTGTGAGTAGGCCCTGAGCGTGAGGGCTCCACCCCAACAGTCCCCCATG7070.1574946981982785No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7000.15593534475077075No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6900.15370769696861689No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6840.15237110829932457No Hit
GGAACATATTGTACAAAAAATCAGAATGTGTTTTAGGAAACTTCCTGTAA6820.1519255787428938No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6790.15125728440824765No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6750.1503662252953861No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6750.1503662252953861No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6740.1501434605171707No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6740.1501434605171707No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG6700.14925240140430918No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6670.14858410706966302No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6660.14836134229144762No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6630.14769304795680147No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6620.14747028317858607No Hit
ATGCAAGACCTTGGGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGG6580.14657922406572452No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6350.14145563416677062No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6300.1403418102756937No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAA6220.1385596920499706No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6110.13610927948960133No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6100.13588651471138596No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6070.13521822037673978No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA6030.13432716126387825No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTCTGATTTTTTGT5960.13276780781637054No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5900.13143121914707823No Hit
GCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGA5890.13120845436886283No Hit
GAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGA5880.13098568959064746No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5850.13031739525600128No Hit
ACCTTGGGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC5810.12942633614313973No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5760.1283125122520628No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5760.1283125122520628No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5680.1265303940263397No Hit
GTCTTGCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5650.12586209969169354No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCACGCATGCGCTGATGGCC5590.12452551102240123No Hit
CTCACGCTCAGGGCCTACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCC5570.12407998146597046No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5540.12341168713132429No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC5510.12274339279667813No Hit
GTTTACAGGAAGTTTCCTAAAACACATTCTGATTTTTTGTACAATATGTT5490.12229786324024734No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5490.12229786324024734No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5450.1214068041273858No Hit
AGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTC5440.12118403934917044No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5360.11940192112344732No Hit
GAGCTTGAGGGCTCCACCCCAACAGTCCCCCATGGGGAGGGGTGAACCCT5350.11917915634523195No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5340.11895639156701655No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5300.11806533245415501No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG5290.11784256767593962No Hit
TCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT5220.11628321422843192No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5210.11606044945021653No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5200.11583768467200115No Hit
ATCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGC5120.11405556644627805No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5110.11383280166806266No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5100.11361003688984726No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC5040.11227344822055496No Hit
CTCAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5030.11205068344233957No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT5000.11138238910769341No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5000.11138238910769341No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4950.11026856521661647No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4940.11004580043840109No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4910.10937750610375493No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4880.10870921176910876No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT4870.10848644699089338No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4870.10848644699089338No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4830.10759538787803181No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4760.10603603443052412No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4740.10559050487409334No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4740.10559050487409334No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4730.10536774009587797No Hit
GGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG4720.10514497531766258No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4690.10447668098301642No Hit
GAGCGTGAGGGCTCCACCCCAACAGTCCCCCATGGGGAGGGGTGAACCCT4660.10380838664837025No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAAA4640.10336285709193949No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4620.1029173275355087No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4590.10224903320086255No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4590.10224903320086255No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4560.1015807388662164No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4560.1015807388662164No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4510.10046691497513946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)