FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328637
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18500.5629311367861805No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17430.5303724169828717No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG16010.4871636486457703No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12970.39466036995225734No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG12570.3824888859136373No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA12140.36940454057212063No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11210.34110584018232887No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10650.32406576252826064No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA10370.3155457237012266No Hit
GTTTATAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10200.31037284298481305No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9640.29333276533074487No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9630.29302847822977934No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9560.2908984685230208No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG9210.2802484199892282No Hit
CCTATAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8720.26533835204191863No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT8640.2629040552341946No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8150.24799398728688493No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8130.24738541308495393No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7700.23430106774343729No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7690.23399678064247179No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7640.23247534513764428No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7470.2273024644212307No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7430.2260853160173687No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7160.2178695642913001No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7140.2172609900893691No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6880.20934952546426605No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6680.20326378344495596No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6640.20204663504109396No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6420.19535231881985293No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG6250.19017943810343935No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGACC6230.18957086390150835No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACA6230.18957086390150835No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6220.18926657680054285No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6190.18835371549764635No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6120.18622370579088782No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6050.18409369608412932No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5990.1822679734783363No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5950.18105082507447426No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5800.17648651855999173No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG5790.17618223145902623No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5710.1737479346513022No Hit
GTATACATCCTTTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGC5690.1731393604493712No Hit
ATTGTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5650.17192221204550917No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG5570.16948791523778514No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5550.16887934103585414No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTATAA5440.1655321829252336No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5410.1646193216223371No Hit
GCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTC5400.1643150345213716No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5400.1643150345213716No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5390.1640107474204061No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5340.16248931191557858No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTTCAGACGCGACATTATT5280.16066358930978558No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5280.16066358930978558No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5230.15914215380495805No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCAGGCGCTGATGGCC5150.15670785699723402No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5100.15518642149240652No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG5100.15518642149240652No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5050.15366498598757902No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT5020.1527521246846825No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4980.1515349762808205No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4940.15031782787695847No Hit
GTATGTAGTTAATCATTACTTTCAGACGCGACATTATTTACACACTCTTT4870.14818781817019994No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4860.14788353106923444No Hit
GTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC4790.1457535213624759No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4580.13936349224220035No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4550.13845063093930385No Hit
GTCGGTACACCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG4530.13784205673737285No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4460.13571204703061432No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4440.13510347282868332No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4420.13449489862675232No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4410.13419061152578682No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4390.13358203732385582No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4360.13266917602095932No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4360.13266917602095932No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4340.1320606018190283No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4290.1305391663142008No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4270.12993059211226976No Hit
CAATAGGCCTGTTTATAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4270.12993059211226976No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4260.12962630501130426No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4250.12932201791033876No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4240.12901773080937326No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4180.12719200820358023No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4170.12688772110261473No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAAGGCTCCCA4170.12688772110261473No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4170.12688772110261473No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGTCTTGTAAGTTGGCGA4160.12658343400164923No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4150.12627914690068373No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4150.12627914690068373No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4120.1253662855977872No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAC4110.1250619984968217No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4110.1250619984968217No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4100.12475771139585622No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4060.12354056299199422No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4060.12354056299199422No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCCTGCGTGGAACCTTTGTGTC3990.12141055328523569No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3980.12110626618427019No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT3950.12019340488137367No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3940.11988911778040817No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3940.11988911778040817No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC3920.11928054357847717No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA3920.11928054357847717No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3900.11867196937654617No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG3890.11836768227558064No Hit
ATCCAGAGTATGTAGTTAATCATTACTTTCAGACGCGACATTATTTACAC3880.11806339517461514No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG3830.11654195966978764No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3830.11654195966978764No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT3820.11623767256882214No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3790.11532481126592563No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA3790.11532481126592563No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3750.11410766286206363No Hit
ATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTCCATGC3750.11410766286206363No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT3730.1134990886601326No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3700.1125862273572361No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAACTAATTATGCCTGCTAGG3670.1116733660543396No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3620.11015193054951207No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3610.10984764344854657No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3590.10923906924661557No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3570.10863049504468456No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG3570.10863049504468456No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC3570.10863049504468456No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3550.10802192084275355No Hit
TCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA3520.10710905953985705No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC3510.10680477243889155No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCCATAG3490.10619619823696053No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3430.10437047563116753No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3410.10376190142923651No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA3400.10345761432827101No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG3400.10345761432827101No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG3390.10315332722730551No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3380.10284904012634001No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA3360.10224046592440901No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA3360.10224046592440901No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA3320.10102331752054698No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC3320.10102331752054698No Hit
GTTCAGATACTGTTTACTTAGAAAGGTCTTGTAAGTTGGCGAGAAAGTAA3300.10041474331861598No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG3300.10041474331861598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)