FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563222
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA25530.45328485037871385No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT21920.38918934274584444No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT20460.3632670598804734No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT19660.34906306926931124No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16220.2879859096413137No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14720.2613534272453846No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13770.24448618839462946No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG13750.24413108862935043No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12510.222114903182049No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12130.21536800764174693No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT11940.21199455987159593No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC11930.2118170099889564No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11680.20737826292296818No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG11520.20453746480073579No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT11450.20329461562225906No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA10710.19015592430693404No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG10390.18447432806246916No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT10300.1828763791187134No Hit
CTCCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9960.17683968310896947No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9870.17524173416521371No Hit
CTCTAAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT9800.17399888498673702No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT9750.17311113557353938No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9620.17080298709922553No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT9610.170625437216586No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC9580.17009278756866741No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9580.17009278756866741No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9220.16370099179364445No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9210.1635234419110049No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC9090.16139284331933057No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8640.15340309860055182No Hit
CCAATCGGCAGTCAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGA8310.14754395247344743No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8280.14701130282552885No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8160.14488070423385452No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8140.14452560446857546No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8000.14203990611162204No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7570.13440526115812237No Hit
ATTTATAGGAAGTTTCCGAAAACAATGTTTGATTTTGTGTACAATATGTT7550.13405016139284331No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7530.13369506162756425No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7520.13351751174492474No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7510.1333399618622852No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC7490.13298486209700616No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGATGGAGTTTG7470.1326297623317271No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG7470.1326297623317271No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7240.12854611503101795No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7210.12801346538309938No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7180.1274808157351808No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7030.12481756749558788No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7010.12446246773030883No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6910.12268696890391356No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC6830.12126656984279734No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6810.12091147007751828No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCGGGTC6800.12073392019487875No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6630.11771557219000678No Hit
CTACTGTACCTGTCTTTAACCCTGACTGGCAAACTCCATCTTTTCCTCAC6510.11558497359833245No Hit
GCTTACTACCAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6490.1152298738330534No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA6480.11505232395041386No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6470.11487477406777434No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTC6460.11469722418513482No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG6450.11451967430249528No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6400.11363192488929766No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6350.11274417547610001No Hit
GTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6330.11238907571082095No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCGGGTCTTTGCCAAGTGTTTGCTG6320.11221152582818142No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6190.10990337735386757No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6150.10919317782330945No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTTTCCATGC6110.10848297829275134No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6080.10795032864483275No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6080.10795032864483275No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6060.10759522887955372No Hit
CTCCACCACATGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATA6060.10759522887955372No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5910.1049319806399608No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTTACTACCAATTCCCTGG5900.10475443075732127No Hit
CTCCCAGTACAAAGACCTTTAATCTAAACTCCTCCCCCAACTCCTCCCAG5890.10457688087468174No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5830.10351158157884457No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCATG5810.10315648181356551No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5780.10262383216564694No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGATGGAGTTTGCCAGTC5770.10244628228300741No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5760.10226873240036789No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5740.10191363263508883No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTGAAGGGAG5710.10138098298717024No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5710.10138098298717024No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5690.10102588322189118No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCCAAACAGGCTTTCACTTT5690.10102588322189118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)