FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563222
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21640.38421794603193765No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT20310.3606038116408805No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT18330.32544893487825405No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT17270.3066286473184641No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15810.2807063644530931No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG13770.24448618839462946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13750.24413108862935043No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13620.24182294015503655No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11960.212349659636875No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG11560.20524766433129388No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11490.2040048151528172No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT10800.1917538732506898No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10280.18252127935343435No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT10220.18145598005759717No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA10060.17861518193536474No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT10050.17843763205272523No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9920.17612948357841135No Hit
CTCCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9700.17222338616034175No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9460.16796218897699308No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9380.16654178991587687No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9290.16494384097212111No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT9190.16316834214572584No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC9180.1629907922630863No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC8930.15855204519709812No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8790.15606634684014473No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT8640.15340309860055182No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8210.14576845364705213No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8050.1429276555248197No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG8030.14257255575954064No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7940.1409746068157849No Hit
ATTTATAGGAAGTTTCCGAAAACAATGTTTGATTTTGTGTACAATATGTT7760.13777870892827337No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7740.13742360916299434No Hit
CTCTAAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT7350.13049916374005277No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCGGGTC7330.1301440639747737No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGATGGAGTTTG7220.12819101526573892No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7170.12730326585254126No Hit
GTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7160.12712571596990174No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7160.12712571596990174No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7110.1262379665567041No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7070.125527767026146No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC7000.12428491784766929No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6970.12375226819975071No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6940.12321961855183215No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6920.12286451878655308No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6900.12250941902127402No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6850.1216216696080764No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG6810.12091147007751828No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6730.11949107101640206No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6650.11807067195528584No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6600.11718292254208819No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC6520.11576252348097199No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6520.11576252348097199No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG6510.11558497359833245No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCGGGTCTTTGCCAAGTGTTTGCTG6420.11398702465457669No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6390.11345437500665811No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6310.1120339759455419No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA6290.11167887618026284No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6290.11167887618026284No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6250.11096867664970474No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6240.11079112676706519No Hit
CCAATCGGCAGTCAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGA6240.11079112676706519No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6100.10830542841011183No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6090.1081278785274723No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6080.10795032864483275No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTC6070.10777277876219324No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6040.10724012911427466No Hit
GCTTACTACCAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6040.10724012911427466No Hit
CTACTGTACCTGTCTTTAACCCTGACTGGCAAACTCCATCTTTTCCTCAC6030.10706257923163513No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGATGGAGTTTGCCAGTC6020.1068850293489956No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5920.10510953052260033No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5910.1049319806399608No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCATG5900.10475443075732127No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCGGGTCTTTGCCAAGTG5710.10138098298717024No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5690.10102588322189118No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG5690.10102588322189118No Hit
ACTTCTCGGCACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT5680.10084833333925167No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5670.10067078345661214No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5660.1004932335739726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)