FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435790
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14440.3313522568209459No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG14440.3313522568209459No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13090.300374033364694No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11530.26457697514858075No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11300.259299203744923No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11010.25264462240987634No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10810.24805525597191308No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10660.24461323114344066No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG10650.2443837628215425No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10480.24048280134927372No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC10110.23199247343904175No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10080.23130406847334722No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9940.228091511966773No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9840.2257968287477914No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9780.2244200188164024No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9670.2218958672755226No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9160.21019298285871635No Hit
TTTTTTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT8800.2019321232703825No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA8780.2014731866265862No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8690.19940797172950273No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8550.1961954152229285No Hit
ATAGTATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG8110.18609880905940934No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8100.18586934073751119No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7830.1796736960462608No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7740.17760848114917735No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7610.17462539296450125No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC7560.17347805135501043No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7500.17210124142362146No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA7380.16934762156084351No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC7350.16865921659514904No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7050.16177516693820418No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6980.16016888868491705No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT6940.1592510153973244No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6860.1574152688221391No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6310.1447945111177402No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6290.14433557447394385No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATACGGCGATGGCC6210.14249982789875856No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6090.13974620803598065No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6030.13836939810459167No Hit
TTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT6000.13768099313889717No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5970.1369925881732027No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5880.13492737327611923No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5870.13469790495422107No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5780.13263269005713763No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5770.13240322173523944No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5760.1321737534133413No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5750.13194428509144313No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5670.13010853851625784No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT5660.12987907019435968No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC5660.12987907019435968No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5590.12827279194107252No Hit
CCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCG5530.12689598200968355No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5500.12620757704398908No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5460.12528970375639642No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5460.12528970375639642No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5450.12506023543449826No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5450.12506023543449826No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5400.12391289382500746No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5350.12276555221551666No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5310.121847678927924No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5280.12115927396222952No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5270.12092980564033136No Hit
GTCCCGTGCTGGTAGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC5260.12070033731843319No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5220.11978246403084053No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5210.11955299570894239No Hit
CATTTGAACCCTAATAAAACCAAGCGCTGGGGCTACTCCCTTAACTTCAT5180.11886459074324789No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5160.11840565409945156No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5130.11771724913375708No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5110.11725831248996077No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACCTCCCCC5100.11702884416806258No Hit
AGTTAAGGGAGTAGCCCCAGCGCTTGGTTTTATTAGGGTTCAAATGTATA5040.11565203423667361No Hit
CCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC5000.11473416094908098No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATC4990.11450469262718281No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4960.11381628766148834No Hit
GGTTGGGGCTTGGCTATTGGCCATCGCCGTATGCGTGGAACCTTTGTGGC4940.11335735101769201No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4900.11243947773009937No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCATCAACTACC4890.1122100094082012No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4880.11198054108630304No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT4840.1110626677987104No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4820.11060373115491406No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4770.10945638954542325No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4770.10945638954542325No Hit
ATTTGGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4720.10830904793593245No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4710.10807957961403428No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC4630.106243833038849No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4600.1055554280731545No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4580.10509649142935817No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4580.10509649142935817No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4560.10463755478556185No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGATCCTGG4560.10463755478556185No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAA4550.1044080864636637No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4550.1044080864636637No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT4540.10417861814176553No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4530.10394914981986737No Hit
GTAGAGTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4520.1037196814979692No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4510.10349021317607104No Hit
AGAGATTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGAC4500.10326074485417287No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4480.10280180821037656No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4450.10211340324468207No Hit
GTTAAGGGAGTAGCCCCAGCGCTTGGTTTTATTAGGGTTCAAATGTATAC4380.10050712499139493No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4360.1000481883475986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)