FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432341
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGTGTGCGGCGCCTGTTGGTGAACTATTGCAGAGCCTCCCAGC29610.6848760584816151No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13100.3030015658935886No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC12630.2921305173462614No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10770.24910892096747708No Hit
GTGCTGGTGACCTGGCTGTCCTGGGCCTCGGGCACAAGGGCAGCTGCGCC10390.24031956256751036No Hit
GGTGTCTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG9600.22204694905179015No Hit
GTACCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG9300.2151079818939217No Hit
GCCTAGTCATCTCGTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9110.21071330269393837No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8740.20215524319923392No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8510.19683536837820145No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7880.18226353734667775No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7830.18110704282036635No Hit
ACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACG7820.18087574391510405No Hit
GTGCTGGTGGGACTGCAGCATTGAGGATGACAAGAGGTCATTTTTGTCGC7740.1790253526730058No Hit
GTTCAGATACTGTTGACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7660.17717496143090755No Hit
ATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7430.17185508660987509No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT7030.16260313039938382No Hit
GTGCTGGTGAGGCTGTGGATAAATTGGAACATTTTACACTGTTGATGGAA6870.15890234791518731No Hit
GGATTATACTTCCTGCCTTCAGGGCATAGCACATACTCTTTTCTTTGCTA6850.15843975010466277No Hit
CAGTAAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCG6530.15103818513626974No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6400.14803129936786008No Hit
GGTCCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG6270.14502441359945042No Hit
CCTCCTTCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6120.1415549300205162No Hit
GTGCTGGTGGGAGTGTAGCACTGAGGATGACCAGAGGTCACTCTCATTGC6060.14016713658894253No Hit
GGGAAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT5980.13831674534684427No Hit
ATATGTGCCTGTGGCAACGTGCCCCAACACCCAATTTCATATCCCATGAA5970.138085446441582No Hit
GATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTATAC5970.138085446441582No Hit
GTGCTGGTGGGAGTGTACCAGTGAGGATAACTAGAGATCGCTCTCATTGC5870.13577245738895918No Hit
ATATAGAATTGGGACTCAAAATATTCGTGGAGTGAATGACCAGATTAATC5820.13461596286264776No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5760.1332281694310741No Hit
ATATAAAAGAGAGTCCACTCGTAGCGCCTCATTTTTCGGGTCACCATATT5740.13276557162054953No Hit
GTATCGGGAGGCCTTGGAGTCTCCGGAGCATTGTTCACCTCACCATACAG5360.12397621322058283No Hit
ATTCTATATTGTACCTGGTCCTAGGCCGGCAGTGCTAGGGACACAGTGAT5340.12351361541005827No Hit
TGATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTATA5290.12235712088374685No Hit
GCACGGGACCATGCAAGACCTGCACAACTCCTGCTCAAGGGACCTCTATG5180.11981283292586177No Hit
ATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTT5040.11657464825218981No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG4860.11241126795746875No Hit
CCTATTGACCTTGCAAATGACATTCCCTTTGTGCGGCACTCCCGTCCTCT4850.11217996905220648No Hit
CCTTCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC4850.11217996905220648No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4810.11125477343115736No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT4810.11125477343115736No Hit
TTCATGGAGAGCTGCTTCCTCTGTGCCTGGCCTCCTGGGACTCACAGTTG4760.11009827890484596No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4750.10986697999958366No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG4720.10917308328379682No Hit
CTGCAAGACAGAGCTGCTGCTGTCATTGTCGCTGTCCCAAGCCAGATCCT4710.10894178437853452No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4550.10524100189433805No Hit
ATACATAGGATTATACTTCCTGCCTTCAGGGCATAGCACATACTCTTTTC4440.10269671393645294No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4380.10130892050487925No Hit
CTCTTTGGAAGGCGGGTGTCTTATATAAAAGAGAGTCCACTCGTAGCGCC4380.10130892050487925No Hit
GTCAATAGGTGGGCATGAGTGGAGTGGAGAGATTAATCTGGTCATTCACT4330.10015242597856784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)