FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293776
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21590.734913675725723No Hit
TCCATAGAGTGTGTAAATAATGTCGGGTTTGGAAGTAATGATTAACTAGA17050.5803741626273079No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC11690.3979222264582539No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGCCCACCGTG10330.3516284516093895No Hit
CCTCCACCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA9260.31520614345623876No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8900.30295190893742174No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8870.30193072272752025No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7850.26721039159087195No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7640.260062088121562No Hit
CTCTAAGACCGTTGATCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA7280.24780785360274493No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7230.24610587658624258No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACC7200.24508469037634117No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC7100.24168073634333642No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6940.23623440989052882No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATATCCC6680.2273841294047165No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG6450.21955503512880561No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6260.2130875224660966No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6240.21240673165949567No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT6220.2117259408528947No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGT6150.20934317302979139No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGTTTGGA5810.19776972931757528No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5650.1923234028647677No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC5590.19028103044496486No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT5320.1810903545558521No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC5280.1797287729426502No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5020.17087849245683787No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG4770.162368607374326No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4720.16066663035782364No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG4560.15522030390501607No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG4530.15419911769511463No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4510.1535183268885137No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAAT4490.15283753608191275No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTG4480.15249714067861228No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4450.15147595446871087No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC4390.14943358204890803No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTAATGACTTCTTTCCTTCAGTACG4390.14943358204890803No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4300.14637002341920377No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4250.14466804640270137No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA4230.14398725559610043No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4210.1433064647894995No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4180.14228527857959805No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGAACAACAGAGT4120.1402429061597952No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC4110.13990251075649476No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4060.13820053373999236No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3980.13547737051358857No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC3940.1341157889003867No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3900.1327542072871848No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA3890.13241381188388432No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3880.13207341648058385No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT3880.13207341648058385No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3840.13071183486738194No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT3740.12730788083437722No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3730.12696748543107675No Hit
GACATATCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG3690.12560590381787484No Hit
CCTCATCATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGATTTAAT3690.12560590381787484No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG3680.12526550841457437No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3670.1249251130112739No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3670.1249251130112739No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3630.123563531398072No Hit
ACCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGAGACTA3630.123563531398072No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGGCCTTT3580.12186155438156963No Hit
GTGTAAATAATGTCGGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3510.1194787865584663No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3480.1184576003485649No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTGTTTGATTTTCTGT3470.11811720494526441No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGTTT3440.117096018735363No Hit
ACTTGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC3420.11641522792876205No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGC3420.11641522792876205No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3400.1157344371221611No Hit
GTACAGTAGAGGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA3380.11505364631556016No Hit
ATGTATACTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3350.11403246010565873No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3350.11403246010565873No Hit
GCCTGAGGATGAGTGTCTCGCAAAGGTGGAGTCAGCGGGGTAGGCTGCCT3340.11369206470235825No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3330.1133516692990578No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3300.11233048308915636No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3300.11233048308915636No Hit
CTATAAGGTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC3270.11130929687925495No Hit
GATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCC3250.110628506072654No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAGGAGGGA3200.10892652905615163No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA3200.10892652905615163No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3190.10858613365285115No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3170.1079053428462502No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3160.10756494744294974No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3160.10756494744294974No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACTG3160.10756494744294974No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3130.1065437612330483No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACG3130.1065437612330483No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG3130.1065437612330483No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT3120.10620336582974783No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3120.10620336582974783No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3100.10552257502314688No Hit
GGATATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATTATACA3060.10416099340994499No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3060.10416099340994499No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTT3040.10348020260334403No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGA3030.10313980720004358No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA3010.10245901639344263No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT3010.10245901639344263No Hit
CCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGAGACTAC2990.10177822558684169No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2970.10109743478024073No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG2960.10075703937694025No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC2960.10075703937694025No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC2960.10075703937694025No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2940.1000762485703393No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCTAATATACATTTACA2940.1000762485703393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)