FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350052
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACACAGCGCC22360.6387622410384742No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC21590.6167655091243587No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA18730.5350633620147864No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG17670.5047821466524973No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17250.49278392924479786No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15690.4482191217304857No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT10050.2871002022556649No Hit
TCCTCAGGCTATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG9700.2771016877492487No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8560.24453509764263598No Hit
ACTACATGTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATA7820.22339538125764172No Hit
GCCTGAGGATGAGTGTTTCTTAAAGGTGGAGACAGAGGGGTCGGCTGCCG7760.22168135019939894No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGACAGTC7520.21482522596642786No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7370.21054014832082088No Hit
GCATAGCCTGAGGATGAGTGTTTCTTAAAGGTGGAGACAGAGGGGTCGGC6920.19768491538400007No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCATGTTCTGCAGGGTCCCGTG6870.19625655616879778No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAACAGTGGTGGGCGTTC6740.19254282220927177No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6730.1922571503662313No Hit
ATGTAAGACCTTGGGCAAACAGTGGTGGGCGTTCACGGTGGTCTCCATGC6690.19111446299406945No Hit
ACAACACACAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGA6660.19025744746494808No Hit
ACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTAT6380.1822586358598151No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6100.17425982425468214No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT5930.1694034029229943No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC5900.1685463873938729No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACCGAGT5870.16768937186475152No Hit
CCTCTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5770.1648326534343469No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGCGTCCAAGGAAT5740.1639756379052255No Hit
GCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGATCTAAT5690.1625472786900232No Hit
AAACAACACACAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAA5630.16083324763178042No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5600.15997623210265904No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5560.15883354473049718No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5540.15826220104441627No Hit
CCCAAAGTGCGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAAC5530.15797652920137578No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCCGTACG5470.156262498143133No Hit
GTATCGGGACGCCTTAGAGTCTCCTGAGCATTGCTCAGCTCACCATACTG5460.15597682630009255No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGG5410.15454846708489026No Hit
GCTCTGAAGGCTGGATCCAACTGGTGGTCGGGAAAGAATCCCAGAGGATT5350.15283443602664748No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC5300.15140607681144516No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5230.14940637391016193No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5190.14826368653800007No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAACAG5080.14512129626455497No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC5070.14483562442151451No Hit
AGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA4990.14255024967719082No Hit
GTATAAAGGGCCTCAAGATGTTGCACAGACTTGGCCCCCAAAACCACATC4990.14255024967719082No Hit
TAACAATACTGCGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTT4950.14140756230502896No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4910.14026487493286713No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4910.14026487493286713No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC4900.13997920308982664No Hit
GGTTTTGGGGGCCAAGTCTGTGCAACATCTTGAGGCCCTTTATACCGCTG4890.13969353124678618No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATA4740.13540845360117926No Hit
GTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGGATGAAGAGGA4710.13455143807205786No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4650.1328374070138151No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4650.1328374070138151No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCTTGCGCTGAACATG4640.13255173517077462No Hit
ATCCCAGAGTGCGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4630.13226606332773416No Hit
GTTCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACA4630.13226606332773416No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA4620.1319803914846937No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4550.12998068858341044No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4530.12940934489732955No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG4480.1279809856821272No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4440.12683829830996537No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT4440.12683829830996537No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG4400.12569561093780354No Hit
CTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACACAGCGCCTC4400.12569561093780354No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC4390.12540993909476306No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCG4390.12540993909476306No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4300.12283889250739889No Hit
CTTTAGGACATGGACATTGATCCCTATAAAGAATTTGGAGCTTCTGTGGA4280.12226754882131799No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4240.12112486144915612No Hit
ATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGATCCCCCC4220.12055351776307521No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGACGCCTTAGAGTCTCCTGA4190.11969650223395381No Hit
CCCATGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTG4190.11969650223395381No Hit
CTTCAAAGCAAACACTGCAAATCCAGATTGGGACTTCAATCCCAACAAGG4190.11969650223395381No Hit
GTTAATTATGTTAACAATACTGCGGGCCTAAAGTTCAGGCAACTATTGTG4150.11855381486179196No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4120.11769679933267058No Hit
GGTATTGGGGGCCAAGTCTGTGCAACATCTTGAGGCCCTTTATACCGCTG4110.1174111274896301No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA4090.1168397838035492No Hit
GCACGGGACCCTGCAGAACATGCACGACTCCTGCTCAAGGAACCTCTATG4060.1159827682744278No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAAGAGGGA4060.1159827682744278No Hit
CCCCAAAAGGCCTCCGTGTGGAGGGGTGAATCCCAGCCCGAATGCTCCAG4050.11569709643138733No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAA4050.11569709643138733No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4040.11541142458834687No Hit
CACCACAAATCCAGATTGGGACTTCAATCCCAACAAGGACACCTGGCCAG4030.1151257527453064No Hit
GGGACCTAGTAGTTAGTTATGTTAACAATACTGCGGGCCTAAAGTTCAGG4020.11484008090226595No Hit
GGCTATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAGATCCCA4000.11426873721618502No Hit
CTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3970.11341172168706362No Hit
ACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACAT3930.11226903431490179No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC3870.11055500325665901No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3850.10998365957057808No Hit
CCCAAAACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGC3850.10998365957057808No Hit
ATCTAAGGTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG3850.10998365957057808No Hit
CCTGTATTCCCATCCCATCATCATGGGCTTTCGGAAAATTCCTATGGGAG3840.10969798772753761No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG3810.10884097219841624No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3810.10884097219841624No Hit
TTTGCAGGATCCAGTATCCAGGGAACAAGTAGTTAATTATGTTAACAATA3810.10884097219841624No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG3790.1082696285123353No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3790.1082696285123353No Hit
GCCCATGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT3760.10741261298321393No Hit
GTCTCTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3740.106841269297133No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC3740.106841269297133No Hit
ATACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCTTTC3710.1059842537680116No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT3700.10569858192497116No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3700.10569858192497116No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTATTAGACGACGAGGC3690.10541291008193068No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC3670.10484156639584975No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGATCTAATGACTCTA3660.1045558945528093No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC3660.1045558945528093No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3650.10427022270976882No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3610.10312753533760699No Hit
GTTGTGGAGTTCCACTGCATAGCCTGAGGATGAGTGTTTCTTAAAGGTGG3580.10227051980848559No Hit
GGGCAAACAGTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG3580.10227051980848559No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3570.10198484796544514No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3550.10141350427936421No Hit
TTTGCAGTGTTTGCTTTGAAGGCTGGATCCAACTGGTGGTCGGGAAAGAA3520.10055648875024281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)