FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350052
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACACAGCGCC20440.5839132471747055No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC18810.5373487367591101No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG18600.5313496280552604No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA18200.5199227543336418No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16800.47992869630797713No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15270.4362209043227864No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT10090.28824288962782674No Hit
TCCTCAGGCTATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8720.24910584713128334No Hit
GCCTGAGGATGAGTGTTTCTTAAAGGTGGAGACAGAGGGGTCGGCTGCCG8240.23539359866534115No Hit
ACTACATGTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATA8090.23110852101973423No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGACAGTC7860.22453806862980358No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7710.22025299098419665No Hit
GCATAGCCTGAGGATGAGTGTTTCTTAAAGGTGGAGACAGAGGGGTCGGC7560.21596791333858967No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7090.20254133671568797No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6400.18282997954589605No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCATGTTCTGCAGGGTCCCGTG6350.1814016203306937No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6230.17797355821420816No Hit
ACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTAT6070.17340280872556077No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5930.1694034029229943No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5850.16711802817867058No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC5710.1631186223761041No Hit
GTATCGGGACGCCTTAGAGTCTCCTGAGCATTGCTCAGCTCACCATACTG5620.16054757578873996No Hit
ACAACACACAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGA5550.15854787288745673No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5500.1571195136722544No Hit
AAACAACACACAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAA5490.15683384182921395No Hit
GCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGATCTAAT5480.1565481699861735No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCCGTACG5470.156262498143133No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5470.156262498143133No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAACAGTGGTGGGCGTTC5460.15597682630009255No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGCGTCCAAGGAAT5430.15511981077097117No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACCGAGT5420.15483413892793071No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC5360.15312010786968794No Hit
CCCAAAGTGCGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAAC5360.15312010786968794No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5290.1511204049684047No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5260.1502633894392833No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT5250.14997771759624284No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC5240.14969204575320239No Hit
GGTTTTGGGGGCCAAGTCTGTGCAACATCTTGAGGCCCTTTATACCGCTG5230.14940637391016193No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA5190.14826368653800007No Hit
ATGTAAGACCTTGGGCAAACAGTGGTGGGCGTTCACGGTGGTCTCCATGC5180.1479780146949596No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5120.14626398363671683No Hit
CCTCTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5100.14569263995063592No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5080.14512129626455497No Hit
ATCCCAGAGTGCGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA5080.14512129626455497No Hit
GTATAAAGGGCCTCAAGATGTTGCACAGACTTGGCCCCCAAAACCACATC5060.14454995257847406No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC5050.1442642807354336No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATA4970.1419789059911099No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4920.1405505467759076No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC4860.1388365157176648No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGG4680.13369442254293648No Hit
TAACAATACTGCGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTT4600.1314090477986128No Hit
GTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGGATGAAGAGGA4560.13026636042645093No Hit
AGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA4550.12998068858341044No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCTTGCGCTGAACATG4530.12940934489732955No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4450.12712397015300583No Hit
ATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGATCCCCCC4420.12626695462388443No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4420.12626695462388443No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC4410.125981282780844No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT4370.12483859540868214No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4370.12483859540868214No Hit
GCTCTGAAGGCTGGATCCAACTGGTGGTCGGGAAAGAATCCCAGAGGATT4360.12455292356564167No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAAGAGGGA4300.12283889250739889No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG4180.11941083039091335No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4170.11912515854787288No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCG4160.11883948670483241No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4140.1182681430187515No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAACAG4120.11769679933267058No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG4100.11712545564658965No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4100.11712545564658965No Hit
CCCATGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTG4090.1168397838035492No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA4090.1168397838035492No Hit
CTTTAGGACATGGACATTGATCCCTATAAAGAATTTGGAGCTTCTGTGGA4050.11569709643138733No Hit
CTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4050.11569709643138733No Hit
GGTATTGGGGGCCAAGTCTGTGCAACATCTTGAGGCCCTTTATACCGCTG4030.1151257527453064No Hit
ACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACAT4020.11484008090226595No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4020.11484008090226595No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGACGCCTTAGAGTCTCCTGA4000.11426873721618502No Hit
CTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACACAGCGCCTC3950.11284037800098272No Hit
GCACGGGACCCTGCAGAACATGCACGACTCCTGCTCAAGGAACCTCTATG3940.11255470615794225No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3920.11198336247186132No Hit
CACCACAAATCCAGATTGGGACTTCAATCCCAACAAGGACACCTGGCCAG3910.11169769062882086No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3910.11169769062882086No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAA3910.11169769062882086No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3850.10998365957057808No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3850.10998365957057808No Hit
CCCCAAAAGGCCTCCGTGTGGAGGGGTGAATCCCAGCCCGAATGCTCCAG3820.10912664404145671No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3810.10884097219841624No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA3810.10884097219841624No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3800.10855530035537578No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGATCTAATGACTCTA3770.10769828482625439No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3770.10769828482625439No Hit
GTTCTGCAGGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACA3760.10741261298321393No Hit
CTTCAAAGCAAACACTGCAAATCCAGATTGGGACTTCAATCCCAACAAGG3730.10655559745409253No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG3720.10626992561105207No Hit
GCCCATGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT3680.10512723823889022No Hit
ATCCAGAACATGTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC3650.10427022270976882No Hit
ACCTCCAATCACTCACCAACCTCTTGTCCTCCAACTTGTCCTGGTTATCG3630.10369887902368792No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACATTGA3600.10284186349456652No Hit
GTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGACAGTAGTCGGAA3570.10198484796544514No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3570.10198484796544514No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA3560.10169917612240467No Hit
ACTCTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGC3550.10141350427936421No Hit
CCCAAAACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGC3550.10141350427936421No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC3550.10141350427936421No Hit
GGGACCTAGTAGTTAGTTATGTTAACAATACTGCGGGCCTAAAGTTCAGG3530.10084216059328328No Hit
GTTCAGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGA3510.10027081690720237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)