FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574727
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21610.37600460740490704No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG20610.3586050420460497No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16860.2933566719503347No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16410.28552686753884887No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT16080.27978501097042596No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT15850.27578311093788876No Hit
CTATATAAGAGAGAAACCACACGCAGCGCTTCATTTTGTGGGTCACCATA14830.25803555427185426No Hit
ATATAAGAGAGAAACCACACGCAGCGCTTCATTTTGTGGGTCACCATATT14810.25768756296467715No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13590.2364600932268712No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA13430.23367616276945402No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13350.23228419754074545No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12520.21784255829289384No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA12430.2162765974105967No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT12130.2110567278029395No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG12030.20931677126705375No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACACACT11940.2077508103847566No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG11830.20583685819528227No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCGCCAATCGGCAGT11710.20374891035221937No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC11500.20009500162685936No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACCACA11470.19957301466609362No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11420.19870303639815076No Hit
GTATAGGCCCCTCACTTTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG11040.19209120156178497No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG10930.19017724937231067No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT10620.1847833841110649No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10430.181477466692882No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10310.17938951884981913No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9560.16633984483067613No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8920.15520412300100744No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT8870.1543341447330646No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA8850.15398615342588742No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC8720.15172420992923596No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8660.15068023600770453No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8630.1501582490469388No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8470.14737431858952163No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8300.1444163924785159No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8000.13919652287085868No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA7990.13902252721727013No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7780.13536861849191006No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT7680.13362866195602432No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7680.13362866195602432No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7680.13362866195602432No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC7630.13275868368808147No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG7620.1325846880344929No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7610.1324106923809043No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC7450.12962676192348715No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7420.12910477496272144No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7390.12858278800195572No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7360.12806080104119No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA7210.1254508662373614No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA7170.12475488362300709No Hit
CATTTGAACCCTAATAAAACTAAACGTTGGGGATACTCCCTTAACTTCAT7130.1240589010086528No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7100.12353691404788708No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT7090.12336291839429851No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7070.12301492708712138No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6970.12127497055123564No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6920.12040499228329277No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6920.12040499228329277No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6920.12040499228329277No Hit
GTTAAGGGAGTATCCCCAACGTTTAGTTTTATTAGGGTTCAAATGTATAC6870.1195350140153499No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6820.11866503574740703No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC6810.11849104009381844No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6670.11605510094357843No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTCGGAAGAGAAACTGTTCT6650.11570710963640128No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATT6640.1155331139828127No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6600.11483713136845843No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6590.11466313571486984No Hit
CTTCAAAGACTGTGTGTTTAAGGACTGGGAGGAGTCGGGGGAGGAGATTA6560.11414114875410411No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6450.11222719656462982No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6390.11118322264309836No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6370.11083523133592123No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATTTACAT6320.10996525306797836No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTAAAAGCCAAA6300.10961726176080121No Hit
ATATTGTACTGAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA6270.10909527480003549No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6270.10909527480003549No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG6230.1083992921856812No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6190.10770330957132691No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC6170.10735531826414978No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA6170.10735531826414978No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT6140.10683333130338404No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC6130.10665933564979546No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6110.10631134434261832No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACCACACGC6060.10544136607467545No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6060.10544136607467545No Hit
GACCTTTAACCTAATCTCCTCCCCCGACTCCTCCCAGTCCTTAAACACAC6050.10526737042108687No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT6050.10526737042108687No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5980.10404940084596687No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACCACAC5930.103179422578024No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5930.103179422578024No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5930.103179422578024No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5900.10265743561725829No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5870.10213544865649256No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5860.10196145300290398No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5840.10161346169572684No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT5760.10022149646701825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)