FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472916
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27120.5734633634725829No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC20930.44257331111656195No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19780.41825609622004756No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16740.35397406727621816No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15160.3205643285488332No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14360.3036480051425623No Hit
CATTTAAACCCTACCAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT14340.3032250970574055No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA12720.268969542159707No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGCGGGTCACCATATT12290.25987701832883636No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10840.22921618215497047No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10630.22477564726082433No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10580.22371837704793243No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC10570.22350692300535402No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10560.22329546896277563No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTAT10370.21927784215378632No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGAGGTAAAAA9930.20997386428033732No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9860.2084936859822886No Hit
CATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCTCT9780.20680205364166154No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC9460.20003552427915317No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGCGGGTCACCATA9440.1996126161939964No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9360.19792098385336931No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9300.19665225959789898No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATATTTAATCT9020.1907315464057042No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8670.1833306549154607No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8610.18206193065999035No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8540.18058175236194166No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8450.17867866597873616No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8410.17783284980842265No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8310.17571830938263877No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC8280.17508394725490362No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8200.17339231491427654No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8130.17191213661622784No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8120.17170068257364945No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7980.16874032597755204No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC7880.16662578555176819No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7780.1645112451259843No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7720.163242520870514No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7670.16218525065762207No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7660.16197379661504369No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT7460.15774471576347596No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7410.15668744555058403No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7410.15668744555058403No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTT7220.15266981874159471No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7110.15034382427323245No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6920.1463261974642431No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6880.14548038129392957No Hit
GCCTGAGGATGAGTGTTTCTCAGAGGTGGAGACAGAGGGGTAGGCTGCCT6840.14463456512361605No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6660.1408283923572051No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6580.13913676001657801No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6550.13850239788884283No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6480.13702221959079414No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA6460.13659931150563737No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6420.13575349533532383No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6400.13533058725016706No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6350.1342733170372751No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6200.13110150639859933No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGAGGTAAAA6160.1302556902282858No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG6120.12940987405797225No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6120.12940987405797225No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6100.12898696597281545No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6060.12814114980250194No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA6050.12792969575992355No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6020.12729533363218837No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5910.12496933916382613No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT5890.12454643107866936No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5880.12433497703609098No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC5850.12370061490835581No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5850.12370061490835581No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTT5780.12222043661030713No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5760.12179752852515034No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG5720.12095171235483679No Hit
GACATATCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGGTAG5700.12052880426968002No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5660.11968298809936649No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5620.11883717192905294No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5550.11735699363100424No Hit
CCCCAAAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAACGCTCCAG5540.11714553958842586No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5430.11481954512006359No Hit
GGATATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA5420.11460809107748524No Hit
ATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5370.1135508208645933No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5340.11291645873685813No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA5310.11228209660912297No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5300.11207064256654457No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5300.11207064256654457No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5290.11185918852396622No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5250.11101337235365266No Hit
CTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC5220.11037901022591749No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5180.10953319405560395No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCTCTCACAAT5160.1091102859704472No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTC5150.10889883192786881No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5130.10847592384271203No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5060.10699574554466332No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5050.10678429150208493No Hit
CTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG5040.10657283745950655No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC5020.10614992937434979No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5020.10614992937434979No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5010.1059384753317714No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4990.10551556724661462No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGC4960.10488120511887947No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT4960.10488120511887947No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4940.10445829703372268No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4910.10382393490598753No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4910.10382393490598753No Hit
GTCTCCACCTCTGAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA4900.10361248086340916No Hit
CCCCAGACCTGCTGCGAGCGAAACAAGCGGCTAGGAGTTCCGCAGTATGG4860.1027666646930956No Hit
AAACAACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAA4850.10255521065051722No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4850.10255521065051722No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4820.10192084852278206No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAGC4810.10170939448020366No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA4760.10065212426731175No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4750.10044067022473337No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGT4740.10022921618215498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)