FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455570
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA28860.6334921087867946No Hit
GTGCTGGTGAACCCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC27800.6102245538556095No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20950.4598634677437057No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT17850.3918168448317492No Hit
GTTGTAAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14420.31652654915819745No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC13990.30708782404460344No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12690.2785521434686217No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11810.2592356827710341No Hit
GCATATAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC11280.24760190530544154No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC11120.24408982154224376No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG10980.24101674824944574No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10570.2320170336062515No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10540.23135851790065193No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10420.22872445507825362No Hit
CATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA9280.20370085826546966No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9190.20172531114867087No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9180.20150580591347103No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9130.20040827973747175No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG8960.19667669073907412No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8830.19382312268147595No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8640.18965252321267861No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC8460.18570142897908115No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC8080.1773602300414865No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT8040.17648220910068704No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC7960.17472616721908818No Hit
CCTATATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7950.1745066619838883No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7930.1740676515134886No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7880.17297012533748932No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7880.17297012533748932No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7510.1648484316350945No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7480.16418991592949492No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTGCTATTCG7460.1637509054590952No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAA7430.1630923897534956No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAACAAAACAG7420.16287288451829576No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7380.16199486357749632No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7370.16177535834229648No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7320.16067783216629716No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7310.1604583269310973No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGA7120.15628772746229996No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7090.15562921175670041No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7090.15562921175670041No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7080.15540970652150055No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6940.1523366332287025No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTGAAACACATTTTGATTTTTTGT6910.15167811752310292No Hit
ATATTAACGTTGACATAGCTGACTATTAATTCCCTGGATGCTGGATCTTC6900.15145861228790305No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6820.1497025704063042No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6810.14948306517110432No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6800.14926355993590448No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTCAGGAAACTTCCTGTAA6720.1475075180543056No Hit
GAATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAATTCAGACA6660.14619048664310644No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6390.14026384529271024No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6340.13916631911671093No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG6270.13762978247031193No Hit
CCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG6240.13697126676471233No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCATCAACAACC6220.13653225629431262No Hit
GTTTACAGGAAGTTTCCTGAAACACATTTTGATTTTTTGTACAATATGTT6220.13653225629431262No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6170.13543473011831333No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6140.13477621441271376No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6080.13345918300151458No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6020.13214215159031542No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5990.13148363588471587No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5980.131264130649516No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG5970.1310446254143161No Hit
TTTCCAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAAT5970.1310446254143161No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5950.1306056149439164No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5950.1306056149439164No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGGTTA5900.1295080887679171No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5870.12884957306231754No Hit
CATGTATACAAACAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5860.1286300678271177No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5840.12819105735671796No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5840.12819105735671796No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5810.12753254165111838No Hit
CCCTATATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTA5800.1273130364159185No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5750.12621551023991923No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5710.12533748929911978No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5680.1246789735935202No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAACCTCCTCC5660.12423996312312048No Hit
CTATTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG5660.12423996312312048No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5490.12050837412472287No Hit
TCATTAACCTAACCTCCTCCCCCAACTCCTCCCACTCGTTAAACACACAG5470.12006936365432316No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5400.11853282700792414No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5360.1176548060671247No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5340.11721579559672499No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5320.11677678512632528No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5280.11589876418552582No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5260.1154597537151261No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5260.1154597537151261No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5250.11524024847992624No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG5240.11502074324472639No Hit
GAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTG5220.11458173277432666No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5140.11282569089272779No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5110.11216717518712821No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5110.11216717518712821No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA5100.11194766995192836No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5080.11150865948152863No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC5080.11150865948152863No Hit
ATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTCCATGC5060.11106964901112892No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTGCTATTCGAGATCTCCTCGACACC5050.11085014377592907No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC5050.11085014377592907No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5030.11041113330552933No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4990.1095331123647299No Hit
CCTTTATATGCATGTATACAAACAAAACAGGCTTTTACTTTCTCGCCAAC4990.1095331123647299No Hit
ACCACTTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAA4980.10931360712953003No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4960.10887459665913032No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4930.10821608095353075No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4860.10667954430713172No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4820.10580152336633229No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG4810.10558201813113244No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4760.10448449195513312No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4740.1040454814847334No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT4740.1040454814847334No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4700.10316746054393397No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4700.10316746054393397No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG4700.10316746054393397No Hit
ATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAACAAAAC4690.10294795530873412No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGCAGGAAA4670.10250894483833439No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATC4670.10250894483833439No Hit
AACCTAACCTCCTCCCCCAACTCCTCCCACTCGTTAAACACACAGTCTTT4650.10206993436793468No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4640.10185042913273483No Hit
GATTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC4610.10119191342713522No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG4580.10053339772153566No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4580.10053339772153566No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4560.10009438725113594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)