FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455570
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACCCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC26970.5920056193340211No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA26500.5816888732796277No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20830.45722940492130737No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT16700.36657374278376537No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12860.2822837324670193No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC12580.2761375858814233No Hit
GTTGTAAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG12230.26845490264942823No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11460.25155299953903903No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11430.25089448383343943No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10980.24101674824944574No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC10490.23026099172465264No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG10330.22674890796145486No Hit
GCATATAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC10020.21994424567025922No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9900.21731018284786094No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9580.21028601532146543No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9430.20699343679346752No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8990.19733520644467367No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8970.19689619597427396No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG8640.18965252321267861No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8510.18679895515508044No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8380.18394538709748226No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8220.1804333033342845No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8180.1795552823934851No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAA8170.17933577715828522No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC8080.1773602300414865No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGA7960.17472616721908818No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7900.17340913580788903No Hit
CATATAAAGGCATCAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA7830.17187259916149No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7820.17165309392629013No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7810.17143358869109027No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7700.16901903110389183No Hit
CCTATATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7530.1652874421054942No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTCAGGAAACTTCCTGTAA7470.16397041069429508No Hit
ATATTAACGTTGACATAGCTGACTATTAATTCCCTGGATGCTGGATCTTC7140.1567267379326997No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7130.15650723269749983No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7080.15540970652150055No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6980.15321465416950195No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6820.1497025704063042No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6810.14948306517110432No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC6750.14816603375990517No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC6730.14772702328950546No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATACAAACAAAACAG6670.1464099918783063No Hit
TTTCCAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAAT6660.14619048664310644No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6640.1457514761727067No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6600.14487345523190728No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6590.14465394999670741No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6420.14092236099830982No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6400.1404833505279101No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6350.1393858243519108No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6350.1393858243519108No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6350.1393858243519108No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTGAAACACATTTTGATTTTTTGT6320.13872730864631122No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6210.13631275105911275No Hit
GAATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAATTCAGACA6200.13609324582391288No Hit
GTTTACAGGAAGTTTCCTGAAACACATTTTGATTTTTTGTACAATATGTT6200.13609324582391288No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6180.13565423535351318No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6130.1345567091775139No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6060.13302017253111487No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6050.132800667295915No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGGTTA6040.13258116206071516No Hit
CTATTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG5960.13082512017911627No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5950.1306056149439164No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5930.1301666044735167No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5910.12972759400311698No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5880.1290690782975174No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTGCTATTCG5880.1290690782975174No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5830.1279715521215181No Hit
CCCTATATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTA5810.12753254165111838No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5800.1273130364159185No Hit
CCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG5800.1273130364159185No Hit
CATGTATACAAACAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5780.1268740259455188No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCATCAACAACC5700.1251179840639199No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5690.12489847882872006No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5690.12489847882872006No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5600.12292293171192134No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT5470.12006936365432316No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5470.12006936365432316No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5460.11984985841912331No Hit
GAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTG5450.11963035318392344No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5400.11853282700792414No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5390.11831332177272427No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG5300.11633777465592553No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG5300.11633777465592553No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5230.11480123800952653No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5140.11282569089272779No Hit
CCTTTATATGCATGTATACAAACAAAACAGGCTTTTACTTTCTCGCCAAC5130.11260618565752793No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG5120.11238668042232806No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT5110.11216717518712821No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5110.11216717518712821No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5100.11194766995192836No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC5040.1106306385407292No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5020.11019162807032946No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5010.10997212283512961No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5000.10975261759992976No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAACCTCCTCC4970.10909410189433018No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4970.10909410189433018No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4950.10865509142393047No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4950.10865509142393047No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4930.10821608095353075No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4900.10755756524793117No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4890.1073380600127313No Hit
GTCTTCGATGAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT4860.10667954430713172No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4840.106240533836732No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4810.10558201813113244No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4760.10448449195513312No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG4740.1040454814847334No Hit
CTTTATATGCATGTATACAAACAAAACAGGCTTTTACTTTCTCGCCAACT4720.10360647101433369No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4710.10338696577913382No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4710.10338696577913382No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4600.10097240819193537No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4590.10075290295673552No Hit
ACCACTTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAA4570.10031389248633579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)