FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430565
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA37810.8781484793236793No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGATC37740.8765227085341354No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA15940.37021123407615575No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA13530.31423826832185614No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA12930.300303090125765No Hit
GTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCGTTGACATACT12470.2896194535087617No Hit
GTGTTCTTGTGGTAAGGACCCATAACTTCCAATGACATAACCCATAAAAT11880.27591652828260543No Hit
GTATGGATCGGCAGAGGAGCCGAAAAGGTTCCACGCATGCGCTGATGGCC11050.2566395317780126No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAAA10930.25385249613879435No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10760.24990419564990188No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAAT10380.24107858279237745No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT9980.23178846399498332No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAACATTCGGTGGGCGTTC9880.22946593429563478No Hit
ATCCAGGATCTAGAGACCAAGTAGTCAGTTATGTCAACACTAATATGGGC9720.22574988677667718No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9710.22551763380674233No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT9620.22342735707732864No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9150.21251146749039054No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC8920.2071696491818889No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8900.2067051432420192No Hit
GTATCGGGACGCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTG8660.2011310719635827No Hit
ATGTAAGACCTTAGGCAACATTCGGTGGGCGTTCACGGTGGTCTCCATGC8350.19393122989560227No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGGACAACAGAGT8230.19114419425638407No Hit
ATACTATGCTGGGGGGAATTAATGACTCTAGCTACCTGGGTGGGTGTTAA7820.18162182248905506No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7780.18069281060931566No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7750.1799960516995111No Hit
TCCTCAGGCCGTGCAGTGGAATTCCACAACCTTTCACCAAACTCTGCAAG7740.17976379872957624No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7660.17790577497009744No Hit
CTTTTACACAATGTGGTTATCCTGCGTTAATGCCCTTGTATGCATGTATT7500.17418972745113978No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7260.1686156561727033No Hit
GCTTTAGGGCATGGACATCGACCCTTATAAAGAATTTGGAGCTACTGTGG7190.16698988538315931No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7070.16420284974394111No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATCGA6910.16048680222498346No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG6760.15700300767596065No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6650.15444822500667726No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6490.15073217748771964No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA6410.1488741537282408No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6400.14864190075830594No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT6380.14817739481843625No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6350.1474806359086317No Hit
ATATTAGTGTTGACATAACTGACTACTTGGTCTCTAGATCCTGGATCGTC6350.1474806359086317No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6340.14724838293869683No Hit
GTCTAACAACAGTACATTCCGGAAGTGTTGATAGGATAGGGGCATTTGGT6290.14608711808902256No Hit
CCCCAAGAGGCCTCCGTGCGGTGGGGTGAAACCCAGCCCGAATGCTCCAG6260.145390359179218No Hit
GGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT6190.14376458838967404No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6110.14190656463019521No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6080.14120980572039063No Hit
GCACGGGACCATGCCGAACCTGCATGACTACTGCTCAAGGAACCTCTATG6040.14028079384065123No Hit
ATGTATATTTGCGGGAAAGGACAACAGAGTTATCAGTCCCGATAATGTTT6010.1395840349308467No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5970.13865502305110727No Hit
ATTTATGCCTACAGCCTCCGAGTACAAAGATCATTAATCTAATCTCCTCC5970.13865502305110727No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCG5920.137493758201433No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5920.137493758201433No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5840.13563573444195418No Hit
CTCTAAATTTTATGGGTTATGTCATTGGAAGTTATGGGTCCTTACCACAA5780.13424221662234506No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5760.13377771068247535No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5750.1335454577125405No Hit
CTTCTAGATACCGCCTCCGCTCTGTATCGGGACGCCTTAGAGTCTCCTGA5710.13261644583280108No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGC5670.13168743395306168No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5650.13122292801319196No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTTCGGCATGGTCCCGTG5610.13029391613345256No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG5570.12936490425371314No Hit
CTTTAGGGCATGGACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGA5510.12797138643410402No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5470.1270423745543646No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5380.12495209782495094No Hit
ATCCTGGATCGTCCAAATTAACACCCACCCAGGTAGCTAGAGTCATTAAT5370.1247198448550161No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA5340.12402308594521153No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG5320.12355858000534183No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5310.12332632703540697No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5310.12332632703540697No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTAGGTTTT5310.12332632703540697No Hit
GAGTTATCAGTCCCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA5290.12286182109553727No Hit
GAGTAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATGCCCAAAGA5270.12239731515566755No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5220.12123605030599328No Hit
ATCTCTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGGAT5200.12077154436612358No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT5180.12030703842625387No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5170.12007478545631903No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG5160.11984253248638417No Hit
GTATGCATGTATTCACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5150.11961027951644931No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG5000.11612648496742652No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4940.11473296714781739No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4840.11241043744846888No Hit
CTATCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT4770.1107846666589249No Hit
GAATACATGCATACAAGGGCATTAACGCAGGATAACCACATTGTGTAAAA4740.11008790774912035No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4730.10985565477918549No Hit
ATGTGGTTATCCTGCGTTAATGCCCTTGTATGCATGTATTCACTCTAAGC4680.10869438992951122No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4640.10776537804977182No Hit
GCATTGTACACCTCACCATACTGCACTCAGGCAAGCAATACTATGCTGGG4610.10706861913996725No Hit
GTTTAATACCCTTATCCAAGGGTAAATATTTGGTAACCTTTGGATAAAAC4600.10683636617003239No Hit
TCACCATACTGCACTCAGGCAAGCAATACTATGCTGGGGGGAATTAATGA4570.10613960726022784No Hit
CTCCGAAAGACACCAAATACTCTAAAACGGTTTGTCTTCCAAAAGTGAGA4540.10544284835042328No Hit
CCCCTATCCTATCAACACTTCCGGAATGTACTGTTGTTAGACAACGAGGC4540.10544284835042328No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4510.10474608944061872No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCGACTCCTCCCAGTCTTTA4490.10428158350074901No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT4480.10404933053081417No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4440.10312031865107474No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA4410.10242355974127018No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT4410.10242355974127018No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4390.10195905380140048No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATACTATGCTGGGGGG4360.10126229489159591No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCG4350.10103004192166107No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4340.10079778895172621No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4340.10079778895172621No Hit
GGGTTACTCTCTAAATTTTATGGGTTATGTCATTGGAAGTTATGGGTCCT4330.10056553598179135No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4330.10056553598179135No Hit
GGTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4330.10056553598179135No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCATGCAGGTTCGGCATGGTCCC4310.10010103004192165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)