FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376489
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA26290.6982939740603311No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18730.4974912945663751No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA18620.4945695624573361No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18130.48155457397161666No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG13220.35113907710451037No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGACCT13020.34582683690625754No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11330.3009384072310214No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA11310.3004071832111961No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG11210.29775106311206967No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT10890.2892514787948652No Hit
ATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC10130.26906496604150454No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9850.2616278297639506No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9810.26056538172430005No Hit
GGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA9670.2568468135855231No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9600.25498752951613457No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9480.2518001853971829No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9480.2518001853971829No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9010.23931642093128883No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8630.2292231645546085No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT8410.22337970033653043No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7950.21116154788054894No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7790.20691175572194673No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7560.200802679493956No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG7400.19655288733535378No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGACCT7050.18725646698841134No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6950.18460034688928495No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6880.18274106281989647No Hit
CCCTTATCTCCACCTCTGAGAGACACTCATCCTCAGGCCATGCAGTGGAA6760.1795537187009448No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6730.17875688267120687No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6690.1776944346315563No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6680.17742882262164367No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6630.17610076257208046No Hit
CGTTAACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTA6630.17610076257208046No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6600.17530392654234253No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6580.17477270252251725No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA6490.1723821944333035No Hit
GCTCACATCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAA6430.17078852237382766No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6370.1691948503143518No Hit
ACAACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6240.1657418941854875No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6220.1652106701656622No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6150.16335138609627373No Hit
GTATGTAGTTAATCATTATTTCCAGACGCGACATTATTTACACACTCTTT6080.16149210202688524No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6070.16122649001697262No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5970.1585703699178462No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCTACAAT5900.15671108584845772No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5840.1551174137889819No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5820.1545861897691566No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTTGTAACATT5810.15432057775924396No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTGCCACGCATGCGCTGATGGCC5760.15299251770968075No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5710.15166445766011755No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5640.1498051735907291No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5620.14927394957090379No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5510.14635221746186475No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5310.14103997726361195No Hit
ATGTTAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC5270.1399775292239614No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5250.13944630520413612No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5230.13891508118431084No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT5220.1386494691743982No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5190.13785263314466029No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC5170.13732140912483498No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5090.13519651304553387No Hit
GGGTAAAGGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5060.13439967701579594No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5050.13413406500588332No Hit
AACCAGGACAGATTGGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGG4990.13254039294640746No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4990.13254039294640746No Hit
TAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAA4960.13174355691666953No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG4860.12908743681754314No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4840.12855621279771787No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4840.12855621279771787No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4770.12669692872832938No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4760.12643131671841673No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4730.12563448068867883No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4660.12377519661929033No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4660.12377519661929033No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4660.12377519661929033No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4570.12138468853007658No Hit
CCTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC4500.11952540446068809No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4500.11952540446068809No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCAGCCATATCGTCAATCTTA4470.11872856843095018No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4460.11846295642103752No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4440.11793173240121226No Hit
CTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC4420.11740050838138698No Hit
GTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4410.11713489637147434No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCTACAATTCG4390.11660367235164905No Hit
CTCAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4360.11580683632191115No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4330.11501000029217322No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4290.11394755225252265No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4280.11368194024261001No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC4270.11341632823269737No Hit
TCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAAGGAGTTT4270.11341632823269737No Hit
GTATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4260.11315071622278473No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4260.11315071622278473No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTGGTCTC4260.11315071622278473No Hit
GGTTGGGGCTTGGCAATTGGCCATCAGCGCATGCGTGGCACCTTTGTGTC4230.1123538801930468No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4220.11208826818313418No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4210.11182265617322154No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4190.11129143215339625No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG4140.10996337210383304No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4140.10996337210383304No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTTAGATACTGTTTACTT4140.10996337210383304No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGC4070.10810408803444457No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4050.10757286401461927No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG4050.10757286401461927No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4020.10677602798488137No Hit
TAACAACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAA4020.10677602798488137No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTA4010.10651041597496873No Hit
GCTTACATCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAA4000.10624480396505608No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3990.10597919195514344No Hit
GCCTGAGGATGAGTGTCTCTCAGAGGTGGAGATAAGGGAGTAGGCTGCCT3980.1057135799452308No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3980.1057135799452308No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3940.10465113190558023No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3930.1043855198956676No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3930.1043855198956676No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG3930.1043855198956676No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT3920.10411990788575497No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3910.10385429587584233No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3890.10332307185601704No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT3880.1030574598461044No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3820.10146378778662857No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3820.10146378778662857No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA3820.10146378778662857No Hit
GTATGTTGTTAATCATTATTTCCAGACGCGACATTATTTACACACTCTTT3780.100401339746978No Hit
ATGTTGTTAATCATTATTTCCAGACGCGACATTATTTACACACTCTTTGG3770.10013572773706536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)