FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376489
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24720.6565928885040466No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18670.4958976225068992No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18130.48155457397161666No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA17390.4618992852380813No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG13360.35485764524328733No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGACCT12750.3386553126386163No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA10980.29164198688407894No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10530.27968944643801014No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT10360.27517404226949527No Hit
ATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC10360.27517404226949527No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10260.27251792217036885No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10160.26986180207124244No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9340.24808161725840597No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9250.24569110916919218No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8940.23745713686190037No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8820.23426979274294868No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8760.23267612068347282No Hit
GGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA8670.2302856125942591No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8470.2249733723960063No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8330.2212548042572293No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG8290.22019235621757874No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT8090.21488011601932594No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7710.2047868596426456No Hit
CGTTAACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTA7550.20053706748404337No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7380.19602166331552845No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7220.19177187115692623No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7010.1861940189487608No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGACCT6920.18380351085954702No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6880.18274106281989647No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6730.17875688267120687No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6580.17477270252251725No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6410.17025729835400238No Hit
CCCTTATCTCCACCTCTGAGAGACACTCATCCTCAGGCCATGCAGTGGAA6360.16892923830443918No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6330.16813240227470125No Hit
GTATGTAGTTAATCATTATTTCCAGACGCGACATTATTTACACACTCTTT6300.16733556624496335No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6300.16733556624496335No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6280.16680434222513804No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6190.16441383413592428No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6170.163882610116099No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6110.16228893805662317No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6010.15963281795749676No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTTGTAACATT6010.15963281795749676No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA5990.1591015939376715No Hit
GCTCACATCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAA5940.1577735338881083No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5920.157242309868283No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5900.15671108584845772No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5840.1551174137889819No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5750.15272690569976813No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5730.15219568167994282No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5720.1519300696700302No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5570.14794588952134058No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCTACAAT5540.14714905349160268No Hit
ACAACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5530.14688344148169002No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5510.14635221746186475No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5450.14475854540238892No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5440.1444929333924763No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTGCCACGCATGCGCTGATGGCC5420.143961709372651No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5400.1434304853528257No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5250.13944630520413612No Hit
CCTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC5240.13918069319422346No Hit
GGGTAAAGGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5220.1386494691743982No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5200.1381182451545729No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5190.13785263314466029No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5170.13732140912483498No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT5160.13705579711492236No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5050.13413406500588332No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCAGCCATATCGTCAATCTTA5030.13360284098605804No Hit
ATGTTAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4970.13200916892658218No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4910.13041549686710632No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC4840.12855621279771787No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4790.12722815274815466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4760.12643131671841673No Hit
GTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4750.1261657047085041No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4720.12536886867876618No Hit
GGTTGGGGCTTGGCAATTGGCCATCAGCGCATGCGTGGCACCTTTGTGTC4650.12350958460937769No Hit
CTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC4620.12271274857963979No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4590.12191591254990186No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4560.12111907652016393No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4540.12058785250033865No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4490.11925979245077545No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG4460.11846295642103752No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4440.11793173240121226No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4430.11766612039129962No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4410.11713489637147434No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4380.11633806034173641No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4370.11607244833182377No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4350.11554122431199848No Hit
TAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAA4300.11421316426243529No Hit
GCCTGAGGATGAGTGTCTCTCAGAGGTGGAGATAAGGGAGTAGGCTGCCT4250.11288510421287208No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4220.11208826818313418No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA4190.11129143215339625No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4190.11129143215339625No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC4180.11102582014348361No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4160.11049459612365833No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG4150.11022898411374568No Hit
GTATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4150.11022898411374568No Hit
GCTTACATCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAA4150.11022898411374568No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGC4140.10996337210383304No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTTAGATACTGTTTACTT4140.10996337210383304No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4120.10943214808400777No Hit
CCCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG4120.10943214808400777No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4090.10863531205426984No Hit
AACCAGGACAGATTGGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGG4070.10810408803444457No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4050.10757286401461927No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT4020.10677602798488137No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4020.10677602798488137No Hit
CTCAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3990.10597919195514344No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG3960.10518235592540554No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG3950.10491674391549287No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3870.10279184783619176No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3870.10279184783619176No Hit
CCTTTTCTCATTTACTGTAAGGGGCCCCACAAATTGCTCACATCTATCAA3850.1022606238163665No Hit
ATGTTGTTAATCATTATTTCCAGACGCGACATTATTTACACACTCTTTGG3830.10172939979654119No Hit
TAACAACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAA3830.10172939979654119No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG3820.10146378778662857No Hit
GTATGTTGTTAATCATTATTTCCAGACGCGACATTATTTACACACTCTTT3810.10119817577671593No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC3810.10119817577671593No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3800.10093256376680329No Hit
CCCCAAAAGACCTACAATTCGTTGACATACTTTCCAATCAATAGGCCGGT3800.10093256376680329No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG3790.10066695175689064No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3780.100401339746978No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCTACAATTCG3780.100401339746978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)