FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324475
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14420.44441020109407503No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA13960.4302334540411434No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13770.42437784112797594No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13750.42376146082132676No Hit
CTTCCAGATCCCAGAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAG11090.3417828800369828No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC9080.27983665921873796No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7990.2462439325063564No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7820.24100469989983822No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7780.23977193928653978No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7260.22374605131366054No Hit
ATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCATGGCCTGAG6960.21450034671392249No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGACTGGTCTGTGCCAAG6800.2095693042607289No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6530.20124817012096463No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6250.1926188458278758No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5860.18059942984821634No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5740.17690114800832113No Hit
GCTCAGAACACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5690.17536019724169813No Hit
CATTTAAACCCTCACAAAACACAAAGATGGGGATATTCCCTTAACTTCAT5670.17474381693504892No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5530.1704291547885045No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5380.16580630248863548No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5310.16364897141536328No Hit
CTTCAAGATCCCAGAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAG5230.16118345018876645No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5220.16087526003544186No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4940.15224593574235304No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4920.15162955543570383No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4910.15132136528237922No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4840.149164034209107No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4760.14669851298251022No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4710.14515756221588721No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4580.1411510902226674No Hit
GTTCAGATACTGTGTACGTAGAAAGGCTTTGTAAGTTGGCGAGAAAGTAA4560.14053470991601819No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4540.13991832960936898No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAAGCC4510.13899375914939519No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4480.13806918868942136No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT4450.13714461822944757No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4380.13498728715617536No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4300.13252176592957857No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA4250.13098081516295554No Hit
CCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT4200.12943986439633254No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4130.12728253332306033No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4130.12728253332306033No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4120.12697434316973574No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4120.12697434316973574No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4090.12604977270976192No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4090.12604977270976192No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA4060.12512520224978813No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4060.12512520224978813No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4000.12327606132984052No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3930.1211187302565683No Hit
GGCCTATTGATTGGAAGGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT3830.11803682872332229No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCCACAATGTCCTCCCGC3830.11803682872332229No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC3800.11711225826334848No Hit
GTATTATCCAGAATATGTAGTTAATCATTACTTCCAGACGCGACATTATT3760.11587949765005008No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3760.11587949765005008No Hit
GGGTAAAGGTTCAGATACTGTGTACGTAGAAAGGCTTTGTAAGTTGGCGA3740.11526311734340088No Hit
CCTGTAAACAGGCCTATTGATTGGAAGGTATGTCAACGAATTGTGGGTCT3740.11526311734340088No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3720.11464673703675168No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT3720.11464673703675168No Hit
CCTTTACCCCGTTGCTCGGCAACGGACTGGTCTGTGCCAAGTGTTTGCTG3710.11433854688342707No Hit
GTTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3680.11341397642345329No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGACTGGTCTGTGCCAAGTG3660.11279759611680405No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3660.11279759611680405No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA3610.11125664535018107No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA3590.11064026504353185No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC3560.10971569458355807No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3540.10909931427690886No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3520.10848293397025965No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3520.10848293397025965No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA3500.10786655366361043No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3460.10663379305031204No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC3450.10632560289698745No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC3430.10570922259033823No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3430.10570922259033823No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA3420.10540103243701364No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3410.10509284228368904No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3400.10478465213036445No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC3380.10416827182371524No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3380.10416827182371524No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3370.10386008167039062No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA3360.10355189151706602No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3350.10324370136374143No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG3340.10293551121041682No Hit
GCTTACATCTATCCACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAA3340.10293551121041682No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCAC3310.10201094075044304No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3300.10170275059711842No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3290.10139456044379382No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG3290.10139456044379382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)