FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354553
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC18240.5144505898977022No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16840.47496425076081716No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12400.34973614664098174No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12240.34522342216819485No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11930.33648001850217035No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG11640.32830070539524414No Hit
GGATATCTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC10640.3000961774403263No Hit
GTACTAAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT9980.28148118899008046No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9960.2809170984309821No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9870.27837869091503953No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA9820.2769684645172936No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC9470.26709687973307233No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT9320.26286620053983467No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT9280.26173801942163794No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8680.24481530264868723No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8420.23748212538040858No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT8150.22986690283258077No Hit
TTTTTGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACAT8020.22620031419844142No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC7880.22225168028475292No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT7680.21661077469376935No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7600.2143544124573759No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7560.2132262313391792No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7330.2067391899095481No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7250.20448282767315465No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7200.20307260127540874No Hit
GAGATATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG6960.19630351456622844No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6910.19489328816848256No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6890.1943291976093842No Hit
GGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC6780.19122669953434324No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6780.19122669953434324No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6610.1864319297820072No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6300.17768852611598265No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5940.16753489605221222No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG5860.1652785338158188No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5630.15879149238618767No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5570.1570992207088926No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5420.15286854151565493No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5410.15258649623610573No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATG5400.15230445095655656No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5350.15089422455881069No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5310.14976604344061395No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5300.14948399816106478No Hit
ATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGACCCAGCATCCAGGGAA5260.14835581704286804No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT5250.14807377176331887No Hit
GTAGAGACCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5140.14497127368827792No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5140.14497127368827792No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5110.14412513784963038No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5000.1410226397745894No Hit
TTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTGT4990.14074059449504023No Hit
GTAATTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA4980.14045854921549106No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4870.13735605114045008No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4850.13679196058135173No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4740.13368946250631075No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4720.1331253719472124No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATGC4690.13227923610856487No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA4640.13086900971081897No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4640.13086900971081897No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG4630.1305869644312698No Hit
CTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTAT4630.1305869644312698No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATT4570.12889469275397472No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4490.12663833051758128No Hit
GTTTTGGAGTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGG4490.12663833051758128No Hit
GGATTAAAGACAGGAACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4480.1263562852380321No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT4470.12607423995848294No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4450.1255101493993846No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4440.1252281041198354No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4410.12438196828118786No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4350.1226896966038928No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4340.12240765132434361No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4290.12099742492659772No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4280.12071537964704854No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4260.12015128908795017No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4220.11902310796975347No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4200.1184590174106551No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4190.11817697213110594No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4100.11563856461516331No Hit
GAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGACCCAGCATCCAGGG4090.11535651933561415No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4080.11507447405606497No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4040.11394629293786825No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4010.11310015709922071No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT4000.11281811181967152No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATTTACAT3870.1091515231855322No Hit
TTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT3860.10886947790598302No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3850.10858743262643383No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG3830.10802334206733549No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3800.10717720622868795No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC3770.10633107039004042No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3750.10576697983094205No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3710.10463879871274535No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC3700.10435675343319617No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG3700.10435675343319617No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3650.10294652703545026No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3630.10238243647635191No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA3620.10210039119680273No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC3620.10210039119680273No Hit
GGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCAACCACGGGGCG3570.10069016479905685No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3570.10069016479905685No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG3560.10040811951950766No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3560.10040811951950766No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3560.10040811951950766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)