FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375428
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10780.2871389454169641No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10550.2810126042809806No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCGGGATTAAAG10510.2799471536486357No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCGGGATTAAAG10130.26982537264135864No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9970.26556357011197884No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT8930.23786185367101015No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8720.23226823785119916No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT8410.2240109954505258No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7950.21175831317855887No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7690.2048328840683167No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7670.20430015875214422No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6890.18352387142141768No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA6750.17979479420821035No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6700.17846298091777915No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6620.17633207965308928No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6360.1694066505428471No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG6310.1680748372524159No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6300.16780847459432968No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6190.16487848535538108No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC6090.1622148587745187No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6070.1616821334583462No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6070.1616821334583462No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG6060.16141577080025996No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6030.16061668282600128No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC5990.1595512321936563No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5890.15688760561279394No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5840.15555579232236275No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5810.15475670434810404No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5780.1539576163738453No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG5550.14783127523786185No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5530.14729854992168936No Hit
AAACTAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAG5490.14623309928934444No Hit
CAATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTA5460.1454340113150857No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5440.14490128599891325No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA5400.1438358353665683No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5390.14356947270848205No Hit
GTATCCAGTTGGCAGCACACCCTAGCAGCCATGGAAAGGAGGTGTATTTC5360.14277038473422335No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA5170.13770949423058482No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5060.1347795049916362No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5040.13424677967546372No Hit
CCCTCATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATG4880.12998497714608392No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4770.12705498790713532No Hit
GGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG4770.12705498790713532No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4770.12705498790713532No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTCTACGTCCCGTCGGCGCTGAAT4700.12519044930053166No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4670.12439136132627293No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT4630.123325910693928No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4620.12305954803584176No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4590.12226046006158305No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC4520.12039592145497938No Hit
GTCAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT4410.11746593221603077No Hit
GTTCAGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG4400.11719956955794453No Hit
TAGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCATTAC4370.11640048158368584No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT4310.1148023056351684No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4290.11426958031899592No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4260.1134704923447372No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT4220.11240504171239225No Hit
GATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC4160.11080686576387484No Hit
ACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTGACGCA4110.10947505247344365No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4100.1092086898153574No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4080.10867596449918493No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC4080.10867596449918493No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4070.1084096018410987No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA4050.10787687652492621No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC4030.10734415120875374No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4010.10681142589258126No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3990.10627870057640879No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC3980.10601233791832254No Hit
GACCCTGCGTTCGGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCC3970.10574597526023632No Hit
CCTTTTTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG3940.1049468872859776No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3930.10468052462789137No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG3910.10414779931171889No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3900.10388143665363266No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3880.10334871133746018No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT3850.10254962336320146No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3780.1006850847565978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)