FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170143
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10230.6012589410084458No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9030.5307300329722645No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7480.4396301934255302No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6420.3773296579935701No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5940.3491180947790976No Hit
ATACAATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5700.3350123131718613No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4600.2703608141386951No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4530.26624662783658454No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4180.24567569632603162No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG3750.22040283761306664No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3690.21687639221125757No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG3580.21041124230794095No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACAAACT3410.20041964700281528No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA3410.20041964700281528No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT3360.19748094250130774No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCATAT3240.1904280516976896No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3030.17808549279135785No Hit
CATGTATACAATCGAAGCAGGCTTTCACGTTCTCGCCAACTTACAAGGCC2790.16397971118412158No Hit
ATTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACA2710.1592777839817095No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC2670.15692682038050346No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT2630.15457585677929742No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC2590.15222489317809137No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGATATTCCCTTAACTTCAT2540.1492861886765838No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG2530.1486984477762823No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG2530.1486984477762823No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG2470.14517200237447325No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA2450.14399652057387022No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG2390.14047007517206117No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2360.1387068524711566No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA2350.1381191115708551No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA2340.1375313706705536No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT2340.1375313706705536No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2310.13576814796964906No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2300.13518040706934756No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA2290.13459266616904603No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGTTTGGTGGAATGTTGTGG2270.13341718436844302No Hit
GCACGGGACCATGCAAGACTTGCACGATTCCTGCTCAAGGAACCTCTATG2220.13047847986693548No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2180.12812751626572944No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2120.12460107086392033No Hit
GGGCACATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATC2060.12107462546211128No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2050.12048688456180977No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2000.11754818006030221No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA1990.1169604391600007No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT1970.11578495735939769No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCTCGCGGCGATGGCC1930.11343399375819165No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC1920.11284625285789013No Hit
ATGTAATTGGATGTTGGGGTACTTTACCACAAGAACATATTGTACAAAAA1860.10931980745608105No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG1850.10873206655577954No Hit
TTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACAC1830.10755658475517652No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA1820.10696884385487501No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG1820.10696884385487501No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATT1820.10696884385487501No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCGAGCGTGGAACCTTTGTGGC1800.10579336205427199No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT1800.10579336205427199No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG1780.10461788025366897No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAACTCTGCTAG1760.10344239845306595No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT1760.10344239845306595No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC1720.10109143485185991No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAAGTCTTGCATGGTCCCGTG1720.10109143485185991No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG1720.10109143485185991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)