FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103805
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4950.476855642791773No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3480.33524396705361015No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT3060.29478348827127787No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT2950.2841866962092385No Hit
GCCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC2630.25335966475603294No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG2220.21386253070661337No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGACAAAGGCATCAAACCTTAT2180.21000915177496268No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC2120.20422908337748663No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC2070.1994123597129233No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC2000.19266894658253456No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA1800.1734020519242811No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG1750.16858532825971773No Hit
ATTTATAGGCAGTTTTCGAAAACATTGTTTGATTTTTTGTACAATATGTT1750.16858532825971773No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT1630.15702519146476565No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC1630.15702519146476565No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG1620.156061846731853No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG1600.15413515726602764No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA1590.153171812533115No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA1520.14642839940272626No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1490.14353836520398824No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA1460.14064833100525023No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA1450.13968498627233755No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC1440.1387216415394249No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG1440.1387216415394249No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT1430.1377582968065122No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA1420.13679495207359954No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT1350.1300515389432108No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA1330.12812484947738548No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC1290.1242714705457348No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC1260.12138143634699677No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG1250.1204180916140841No Hit
GTTTCGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG1250.1204180916140841No Hit
ATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTTGTACAATATGTT1250.1204180916140841No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC1230.11849140214825876No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG1220.11752805741534608No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG1220.11752805741534608No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG1210.11656471268243342No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG1210.11656471268243342No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT1190.11463802321660807No Hit
TCTTATGTCTTTGGGTATACAGTTAAACCCTAATAAAACCAAACGTTGGG1180.1136746784836954No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA1100.105967920620394No Hit
GTAAACCATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTG1090.10500457588748134No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCGC1090.10500457588748134No Hit
CATAGCCTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCC1070.10307788642165598No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT1070.10307788642165598No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC1060.10211454168874332No Hit
GTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG1050.10115119695583064No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGTTCTTCTGCGAC1040.10018785222291797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)