FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575684
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32370.5622876439157594No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18510.32153056190548984No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT17500.3039862146594312No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14400.2501372280626177No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14170.24614198066995086No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC13930.24197302686890723No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG13270.23050840391603727No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12280.21331146948673232No Hit
GGTTTATATGTATACCCAAAGACAAAAGAAAAGTGGTAACAGCGGCATAA11360.1973304799160651No Hit
CATATAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT10600.1841287928794269No Hit
ATCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGC10210.177354242952731No Hit
TCCACAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10120.17579088527733966No Hit
CTCCTAGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA10050.17457494041870195No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9470.16449996873284647No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9150.15894136366478834No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAACACCCGTG9110.15824653803128105No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8950.15546723549725197No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8850.1537301714134838No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8800.1528616393715997No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAGCATT8710.15129828169620835No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT8010.13913883310983108No Hit
AGTCTAGATTCTGTGGTATTGTGCGGATTTTTGTCAACAAGAAAAACCCC7810.13566470494229474No Hit
GTTTATATGTATACCCAAAGACAAAAGAAAAGTGGTAACAGCGGCATAAA7750.13462246649203383No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA7740.134448760083657No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7360.12784791656533795No Hit
ATGCTGGATCGTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATTAAC7350.12767421015696112No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC7080.12298413713078703No Hit
CAGTAAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCG7060.1226367243140334No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6970.12107336663864203No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6840.1188151833297434No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6810.11829406410461295No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6710.11655700002084478No Hit
ATACCACAGAATCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGA6650.11551476157058385No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6630.11516734875383022No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC6600.11464622952869978No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6540.11360399107843885No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATACCTATGGGAG6300.10943503727739523No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6300.10943503727739523No Hit
GGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCT6210.10787167960200388No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT6190.10752426678525023No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6180.10735056037687342No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6160.10700314756011979No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG6100.10596090910985888No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGT6080.10561349629310524No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6070.10543978988472842No Hit
CTGTGGTATTGTGCGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA6040.10491867065959798No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6020.10457125784284435No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGG6020.10457125784284435No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA6020.10457125784284435No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG6010.10439755143446752No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6000.1042238450260907No Hit
GAATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5960.10352901939258342No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5940.1031816065758298No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5900.10248678094232254No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5890.10231307453394571No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5820.10109712967530798No Hit
GTCCAAGGAATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGAC5800.10074971685855436No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG5790.10057601045017754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)