FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575684
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32390.562635056732513No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT17010.29547460064896713No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16290.2829677392458363No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13980.24284155891079132No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG13400.23276658722493593No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG13320.23137693595792133No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC12870.22356014758096454No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12510.21730671687939912No Hit
GGTTTATATGTATACCCAAAGACAAAAGAAAAGTGGTAACAGCGGCATAA10550.18326026083754282No Hit
CTCCTAGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA10260.17822277499461509No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10070.17492235323545557No Hit
CATATAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT9850.17110081225116558No Hit
ATCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGC9810.1704059866176583No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9340.16224178542394785No Hit
TCCACAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9140.15876765725641148No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9110.15824653803128105No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8670.15060345606270106No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAGCATT8560.14869268557055607No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAACACCCGTG8400.14591338303652698No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT8340.14487114458626607No Hit
GTTTATATGTATACCCAAAGACAAAAGAAAAGTGGTAACAGCGGCATAAA7570.1314957511412511No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA7470.12975868705748295No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7450.1294112742407293No Hit
AGTCTAGATTCTGTGGTATTGTGCGGATTTTTGTCAACAAGAAAAACCCC7380.12819532938209158No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7240.12576343966481612No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6840.1188151833297434No Hit
ATGCTGGATCGTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATTAAC6800.11812035769623613No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC6710.11655700002084478No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6620.11499364234545341No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6550.11377769748681568No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6370.11065098213603297No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG6340.11012986291090252No Hit
ATACCACAGAATCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGA6320.10978245009414886No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGG6310.10960874368577204No Hit
CAGTAAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCG6210.10787167960200388No Hit
GTCCAAGGAATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGAC6150.10682944115174298No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6120.10630832192661252No Hit
GGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCT6050.1050923770679748No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6040.10491867065959798No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6040.10491867065959798No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5940.1031816065758298No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5940.1031816065758298No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATACCTATGGGAG5910.10266048735069935No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG5860.10179195530881524No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGT5860.10179195530881524No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5860.10179195530881524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)