FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366626
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAAATC47571.297507541745539No Hit
ACAGACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG32680.8913715884852683No Hit
GTTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA31150.8496396873107744No Hit
ATTGTCTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA28780.7849961541189113No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG25320.6906220508092715No Hit
TCCACAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTATG23580.6431622416304353No Hit
GCCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC23550.6423439690583865No Hit
GCCTAGTCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC22560.6153409741807727No Hit
CTCTATGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC19480.5313316567837524No Hit
TCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG18840.5138751752467091No Hit
ACATAGAACAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG17540.4784166971245902No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA15890.4334117056619007No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACAGACT15470.4219558896532161No Hit
CTTTCCCGATCACCAGTTGGACCCTACATTCAGAGCCAATTCAAACAATC15160.4135004064087107No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAAATCATT14080.38404259381495043No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14070.3837698362909341No Hit
ATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCA13130.35813062903340187No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12680.3458565404526684No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA12570.34285620768848907No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC12040.3284000589156252No Hit
TCGCCAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCC11210.3057611844222723No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT10690.2915777931734247No Hit
CATATAAAGAATTCGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT9530.25993792038753394No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG9370.25557380000327307No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA9200.25093692209499596No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9130.2490276194268819No Hit
CTCTAAAGCCTCCCGATACAGAGCAGTGGCGGTGTCGAGGAGATCACGAA9120.2487548619028656No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG9040.2465728017107352No Hit
GACTTCTTTCCGTCTATTCGTGATCTCCTCGACACCGCCACTGCTCTGTA8800.240026621134344No Hit
CTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8790.23975386361032772No Hit
CCTTCCATGCCTCTGGCCATGGATCCTTGTTGGGGTTTAAGTCCCAATCT8670.2364807733221321No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8360.22802529007762679No Hit
CCACAATAGTTGTCTAATTTTTAGGCCCATATTAACATTGACATAGCTTA8290.2261159874095127No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTATG8230.22447944226541486No Hit
CATAGAACAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG7970.2173877466409911No Hit
CATGTATACAAGCTAAACAAGCTTTCACTTTTTCGCCAACTTACAAGGCC7890.21520568644886068No Hit
GTCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAAGCC7690.2097505359685347No Hit
ACTCTTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTC7660.2089322633964858No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7650.20865950587246948No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA7600.207295718252388No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7530.20538641558427387No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAAGCCTCCCGATACAG7250.19774920491181747No Hit
CCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCC7180.1958399022437034No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG6890.18792993404723068No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6830.1862933889031329No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC6730.1835658136629699No Hit
GCTATTGTCTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG6630.18083823842280689No Hit
ATCGCCAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGC6530.1781106631826439No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAAATCATTAATCTAGTCTCCTCC6510.1775651481346113No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6490.1770196330865787No Hit
TCCCGATACAGAGCAGTGGCGGTGTCGAGGAGATCACGAATAGACGGAAA6450.1759286029905135No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTATGTGTTCAGG6090.1661093321259267No Hit
CCGAACATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT5890.1606541816456007No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG5850.1595631515495355No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTC5850.1595631515495355No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5830.15901763650150288No Hit
GTCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGG5810.1584721214534703No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5770.1573810913574051No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACACATAT5770.1573810913574051No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5750.1568355763093725No Hit
CTTTAGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGAC5710.1557445462133073No Hit
GAAATGAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGATTTAAATGTA5660.15438075859322578No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5550.15138042582904648No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG5530.1508349107810139No Hit
GCTTTGGGGCATGGACATTGACACATATAAAGAATTCGGAGCTTCTGTGG5500.150016638208965No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5420.1478345780168346No Hit
CTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCT5350.1459252753487205No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5320.14510700277667157No Hit
CATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGC5290.14428873020462268No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5270.14374321515659008No Hit
CTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAGGA5230.1426521850605249No Hit
GACATTGACACATATAAAGAATTCGGAGCTTCTGTGGAGTTACTCTCTTT5230.1426521850605249No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5210.1421066700124923No Hit
GACTTCTTTCCGTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTA5180.1412883974404434No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5010.13665151953216628No Hit
GTACAAAAATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTAG4980.1358332469601174No Hit
TCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCC4970.13556048943610108No Hit
AACATAGAACAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCC4950.1350149743880685No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4800.130923611527824No Hit
GGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTCAAATGAATG4760.1298325814317588No Hit
CCTCTACGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4730.12901430885970988No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG4720.1287415513356936No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4640.12655949114356319No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACCAGACATTATTTACAT4630.1262867336195469No Hit
GCACTAAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTC4610.1257412185715143No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC4590.1251957035234817No Hit
CTTTGGCGATCACCAGTTGGACCCTACATTCAGAGCCAATTCAAACAATC4590.1251957035234817No Hit
CTCCCACCTGGTGTGCCGCTGGCCATGGATCCTTGTTGGGGTTTAAGTCC4590.1251957035234817No Hit
ATACAGAGCAGTGGCGGTGTCGAGGAGATCACGAATAGACGGAAAGAAGT4560.1243774309514328No Hit
GAATTCGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTT4540.1238319159034002No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4520.1232864008553676No Hit
CCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGC4500.122740885807335No Hit
GACCAATTTATGCCTACAGCCTCCCAGTACAAAAATCATTAATCTAGTCT4470.1219226132352861No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGGGCAAC4470.1219226132352861No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4470.1219226132352861No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC4450.1213770981872535No Hit
GTGCTGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAAGCCTCCCGA4430.12083158313922089No Hit
CTCTTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4380.1194677955191394No Hit
GGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAAT4350.1186495229470905No Hit
CCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT4320.1178312503750416No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4300.11728573532700899No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4250.11592194770692749No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG4200.114558160086846No Hit
TCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4140.1129216149427482No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4090.11155782732266668No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4090.11155782732266668No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA4070.11101231227463411No Hit
CTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATT4040.11019403970258519No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4040.11019403970258519No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCAACAAGCTCTGCTAG4000.10910300960652No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACCAGACATTATT3950.10773922198643848No Hit
CTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTCAGGATTAAAG3940.1074664644624222No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC3900.10637543436635699No Hit
GCCTCTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA3870.1055571617943081No Hit
CACACCAGGTGGGAGTGGGATCCTTCGGGCCAGGGTTCACTCCACCACAC3850.1050116467462755No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT3850.1050116467462755No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG3840.1047388892222592No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3830.1044661316982429No Hit
AACAGACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCC3790.10337510160217768No Hit
CTAAAGCCTCCCGATACAGAGCAGTGGCGGTGTCGAGGAGATCACGAATA3780.10310234407816139No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3770.1028295865541451No Hit
CTACTGTTGTTAGACGACGAGGCAGGACCCCTAGAAGAAGAACTCCCTCG3760.10255682903012879No Hit
ACATATTGTTGACATCTATTAATAATATCCTCTTTCAAATGAATGTGAGG3720.10146579893406359No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA3720.10146579893406359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)