FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648464
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC39740.6128327863998618No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA24420.3765822003997138No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC23680.3651706185694194No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20830.32122060746625875No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT20700.31921587011769353No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA20520.316440079942757No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGCTCACCCGTG19490.3005563917195095No Hit
GTATAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTACGTAACCC18810.29007007328086065No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT18770.2894532310197636No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT18590.28667744084482716No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG18550.2860605985837302No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTACGTAACCCATGAA18400.28374744010461644No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT16680.2572232228774458No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC15220.23470848034740557No Hit
GTATACATGCATACAAGGGCATTAAGGCAGGATATCCACATTGTGTAAAT14660.22607268869204766No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG14270.220058476646352No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC14200.21897900268943227No Hit
ATATTATACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG13900.21435268573120483No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC13190.2034037355967332No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT13160.20294110390091047No Hit
CTTATCAACACTTCCGGAAACTACTGTTATTAGATGTCGGGGCCGAGGCA12280.18937057415677663No Hit
CTCCAAAAGAGACCAAATATTCAAGTACCGTCTCTCTTCCAAAAGTAAGG12180.18782846850403415No Hit
GTACATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG11840.1825853092847097No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT11840.1825853092847097No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT11620.17919267684867626No Hit
CATAATTGACTACTAGATCCCTGGATGCTGGATCTTGCAAATTATTACCC11380.1754916232820943No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG11100.17117372745441536No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTAT11090.1710195168891411No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT11000.16963162180167288No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11000.16963162180167288No Hit
GGATCATATTATACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10930.16855214784475314No Hit
GCCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC10900.1680895161489304No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG10840.1671642527572849No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATCAGAGGACAAACGGGCAAC10800.1665474104961879No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA10670.1645426731476227No Hit
GTCAACTATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTG10580.16315477806015447No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10530.16238372523378322No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT10420.16068740901576647No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC10300.15883688223247552No Hit
GTATAAAGGGCCTCACGATGCTGTACAGATTTGGCCCCCAATACCACATC10200.15729477657973304No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC10000.15421056527424806No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA9990.15405635470897383No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG9970.15374793357842534No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA9900.15266845962150558No Hit
TATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCT9480.14619161587998716No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG9360.14434108909669618No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA9090.1401774038342915No Hit
GTGCAGAGGTGAATCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG9080.14002319326901724No Hit
GTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATACAAGCTAAACAG8910.13740161365935502No Hit
GTTGACAAGATAGGGGCATTTGGTGGTCTATAGGCTGGAGGAGTGCGAAT8750.13493424461496706No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8660.13354634952749883No Hit
ATTAACAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG8650.13339213896222457No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8640.13323792839695034No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT8480.13077055935256235No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT8470.13061634878728812No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT8420.12984529596091687No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAGATACAGACCCCT8380.1292284536998199No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT8370.12907424313454563No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8290.12784055861235166No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATCGA8180.12614424239433492No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG8120.12521897900268944No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA8080.12460213674159244No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8070.12444792617631818No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA8000.12336845221939845No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAGCATCGTGAGGCCCTTTATACC7930.12228897826247873No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG7880.12151792543610748No Hit
ATTGACCACAGTGTCAACAGTTCCTCCTCCTGCCTCCACCAATCGGCAGT7760.1196673986528165No Hit
GTACAGATTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7720.11905055639171952No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAA7700.11874213526117101No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAATTGC7680.11843371413062251No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT7670.11827950356534828No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGTACTGTTTACTT7650.11797108243479977No Hit
ATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATACAAGCTAAAC7640.11781687186952552No Hit
GTACTTGAATATTTGGTCTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC7620.11750845073897702No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG7610.11735424017370279No Hit
TTCAAGTACCGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7590.11704581904315428No Hit
AATCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT7590.11704581904315428No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG7580.11689160847788004No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTGCCGATGAGCTTTGC7570.1167373979126058No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT7530.11612055565150879No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGCCTC7480.11534950282513755No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7460.11504108169458907No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7450.1148868711293148No Hit
GGCTGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAAGTA7450.1148868711293148No Hit
GTATTAACATAGTTGACTACTAGATCCCTGGATGCTGGATCTTGCAAATT7380.11380739717239508No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT7350.11334476547657232No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT7300.11257371265020108No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA7290.11241950208492685No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC7220.11134002812800711No Hit
CCTGTTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7170.11056897530163587No Hit
TTATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG7160.11041476473636162No Hit
CTGTTGACACTGTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAA7080.10918108021416764No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG7050.10871844851834489No Hit
ATACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT7040.10856423795307064No Hit
GTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC7020.10825581682252215No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG7000.10794739569197365No Hit
GTTATTAGATGTCGGGGCCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTC6990.1077931851266994No Hit
TCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTG6950.10717634286560242No Hit
CTTTTGTATAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTACGT6870.10594265834340842No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTGCCGATGAGCTTT6860.10578844777813416No Hit
GCCTTAGAGTCTCCTGAGCATTGCACCCCTCACCATACTGCACTCAGGCA6830.10532581608231142No Hit
TCTTATTGGTTCTTCTGGATTATCAAGGTATGTTGCCCGTTTGTCCTCTG6690.10316686816847197No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6630.10224160477682648No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6610.10193318364627797No Hit
GTTGACACTGTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAT6600.10177897308100373No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACGGTACTTGAATATTTGGT6600.10177897308100373No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6590.10162476251572947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)