FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457887
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA26260.573503943112602No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24620.5376872459799034No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17460.381316787766414No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC16240.35467265941160153No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG15010.3278101365620775No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC14130.30859142102745873No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT13840.302257980680823No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGAA13800.3013844027019767No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG13040.28478642110389685No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12610.275395457831299No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCTC12460.2721195404106253No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG11740.25639513679139175No Hit
GTGTAAAGGGAGCGGCAAATCCTAGAAGCCCCACAATCCGTTGACATACT11600.2533376138654297No Hit
GCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATTTGGCCAC11510.2513720634130255No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC11080.24198110014042765No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11040.24110752216158132No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10660.2328085313625414No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9770.21337142133321102No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC9270.20245169659763215No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9100.19873899018753535No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9020.19699183422984273No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT8740.19087678837791858No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8690.18978481590436067No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8520.18607210949426387No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT8480.18519853151541754No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8110.1771179352110892No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8100.17689954071637762No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7940.17340522880099238No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7890.1723132563274345No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7850.17143967834858817No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7780.16991091688560717No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7750.16925573340147243No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7670.16750857744377978No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7620.1664166049702219No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7540.1646694490125293No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7310.159646375634163No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7080.15462330225579674No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7060.1541865132663736No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7050.153968118771662No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7010.15309454079281568No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6970.15222096281396938No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6940.15156577932983467No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6920.1511289903404115No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6840.14938183438271888No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6830.1491634398880073No Hit
CTATCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATGGCCTGAGGA6730.14697949494089155No Hit
GTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGC6680.14588752246733364No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6660.1454507334779105No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGGATTGTGGGGCT6600.14414036650964102No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6470.1413012380783905No Hit
ATTGACAACAGTACCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6380.1393356876259863No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6300.1375885316682937No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG6270.13693334818415898No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6210.1356229812158895No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC6170.13474940323704318No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5980.13059990783752323No Hit
ATCCTATCCGTACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT5970.13038151334281164No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5950.1299447243533885No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5920.1292895408692538No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA5900.1288527518798306No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC5890.12863435738511905No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5870.12819756839569588No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5850.12776077940627273No Hit
TACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGC5850.12776077940627273No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5830.12732399041684958No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5800.12666880693271484No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5780.12623201794329167No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5760.12579522895386852No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA5730.12514004546973378No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5720.12492165097502221No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5720.12492165097502221No Hit
TTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATA5660.12361128400675277No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5600.1223009170384833No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5600.1223009170384833No Hit
GTTCTATCCTATCCGTACTAAATATTTGCCCTTAGATAAAGGCATTAAAC5600.1223009170384833No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5580.12186412804906013No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5580.12186412804906013No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5550.1212089445649254No Hit
ATTTAGTACGGATAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTC5530.12077215557550225No Hit
GACAAAGGATGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC5510.12033536658607911No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5490.11989857759665595No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG5490.11989857759665595No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5480.11968018310194438No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG5430.11858821062838648No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5410.11815142163896332No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5400.11793302714425175No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5390.11771463264954017No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGGATTGTGGGGCTTCTAG5350.11684105467069386No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG5340.11662266017598227No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATCCGTTGACATACT5340.11662266017598227No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5340.11662266017598227No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5330.1164042656812707No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5320.11618587118655913No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5310.11596747669184754No Hit
GGATAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG5300.11574908219713599No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5290.1155306877024244No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5210.11378353174473178No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5210.11378353174473178No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5170.11290995376588547No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5120.11181798129232758No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5120.11181798129232758No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5110.111599586797616No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5080.11094440331348128No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5080.11094440331348128No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5060.11050761432405813No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT5020.10963403634521181No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5010.10941564185050023No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC5000.10919724735578866No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4980.1087604583663655No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4970.10854206387165392No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4950.10810527488223076No Hit
ATAATATCCTCTTTCAAATGAATGAGAGGAAAAGAGGGAGTTTGCCAGTC4940.10788688038751919No Hit
CTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTCCC4900.10701330240867288No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGAGAGG4840.10570293544040342No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4810.10504775195626868No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4800.10482935746155711No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATAC4790.10461096296684554No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4780.10439256847213395No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4780.10439256847213395No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC4740.10351899049328764No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT4730.10330059599857606No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4710.1028638070091529No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4700.10264541251444133No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT4690.10242701801972975No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4690.10242701801972975No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4680.10220862352501817No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC4680.10220862352501817No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC4680.10220862352501817No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4670.10199022903030662No Hit
CCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGG4640.10133504554617188No Hit
GATAATATCCTCTTTCAAATGAATGAGAGGAAAAGAGGGAGTTTGCCAGT4610.10067986206203715No Hit
CTGCTAGATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAG4600.10046146756732556No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4590.10024307307261399No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4580.10002467857790241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)