FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553596
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA26690.4821205355530026No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATGCGTTGACATACT23030.416007341093505No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG21140.38186692100376446No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT20030.3618161980939169No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG17660.31900519512424225No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC16900.30527677223101324No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG16050.2899226150477966No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15290.2761941921545676No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT14740.2662591492713097No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGTTACTCCCTTAATTTCAT14230.25704665496137974No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA14180.25614346924472No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGGTG13730.24801479779478175No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG12520.22615770345161454No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12320.2225449605849753No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA11990.21658393485502062No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC11930.21550011199502886No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA10940.19761703480516477No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10730.19382365479519362No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10230.1847917976285956No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG10180.18388861191193578No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG10090.18226287762194815No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9860.17810822332531304No Hit
ATTTATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAATATGTT9800.17702440046532128No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT9680.17485675474533774No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9610.17359229474201404No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9610.17359229474201404No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC9600.17341165759868207No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC9540.17232783473869032No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG9390.1696182775887109No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9170.16564426043540778No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG9120.16474107471874797No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9070.16383788900208818No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8780.1585994118454613No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC8760.15823813755879737No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8660.1564317661254778No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG8560.15462539469215816No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG8460.1528190232588386No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC8400.15173520039884683No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8130.1468579975288839No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT8130.1468579975288839No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC8090.14613544895555602No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGCATTGTGGGGCT8070.14577417466889211No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7970.1439678032355725No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG7890.14252270608891685No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7720.13945187465227352No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT7610.13746486607562192No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC7580.13692295464562604No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA7530.13601976892896625No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7440.13439403463897862No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT7370.13312957463565486No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT7320.13222638891899507No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7190.1298781060556796No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7190.1298781060556796No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7170.12951683176901568No Hit
CTTTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG7080.12789109747902802No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG6980.12608472604570842No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6980.12608472604570842No Hit
GGGCACATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATC6870.12409771746905686No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6860.1239170803257249No Hit
GAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGT6860.1239170803257249No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6800.12283325746573315No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6790.12265262032240118No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG6780.12247198317906921No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG6770.12229134603573726No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6700.12102688603241353No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6690.12084624888908156No Hit
CTCCTACCTGGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC6610.11940115174242588No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6570.11867860316909803No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT6560.1184979660257661No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6520.11777541745243825No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6410.11578840887578667No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6370.11506586030245883No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6330.11434331172913098No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6320.11416267458579904No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6260.11307885172580727No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6260.11307885172580727No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6210.11217566600914745No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6180.11163375457915158No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6150.1110918431491557No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6150.1110918431491557No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC6150.1110918431491557No Hit
GGTCTATTTATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAAT6100.1101886574324959No Hit
GCACTAAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6050.1092854717158361No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5990.10820164885584434No Hit
TTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATA5980.10802101171251237No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGC5880.10621464027919278No Hit
ATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5870.10603400313586081No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5830.10531145456253296No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5780.10440826884587316No Hit
CTTTGTGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG5760.10404699455920925No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5710.10314380884254944No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC5690.10278253455588551No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5690.10278253455588551No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5660.10224062312588963No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC5650.10205998598255767No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5650.10205998598255767No Hit
ATACAGCACTAAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGAATCTG5640.10187934883922573No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGCATTGTGGGGCTTCTGG5590.10097616312256591No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5570.10061488883590199No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5540.10007297740590611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)