FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552642
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19300.3492315097296261No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA17280.312679818037717No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17000.30761324691210584No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16910.30598470619315943No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16080.29096594178509777No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG15930.2882517072535204No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC15010.27160440212651227No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG14770.2672616268759884No Hit
GGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTT14640.2649092902819547No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT14510.2625569536879209No Hit
GGTTGTAGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGT14000.2533285562805577No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13880.25115716865529586No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA13410.24265256712301997No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12690.2296242413714484No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12590.22781475168373017No Hit
GGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGT12480.22582431302724004No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12160.2200339460265416No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG11990.21695781355742053No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11570.20935795686900383No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11530.2086341609939165No Hit
TCTACAACCAGCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGG10780.19506298833602947No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10750.194520141429714No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG10720.1939772945233985No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10470.18945357030410284No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10380.1878250295851564No Hit
GTACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA10250.18547269299112265No Hit
ATTGACAACAGTGCCAGCAGCCCCTCCTCCTGCCTCCACCAATCGGCAGT10240.18529174402235082No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG10010.1811299177405988No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG9680.1751586017711285No Hit
TTCGTAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGA9610.1738919589897257No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA9580.17334911208341025No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9460.17117772445814833No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9350.1691872858016582No Hit
GTTTTGTGCCCATATTAACATTGACATAGCCGACTACTAATTCCCTGGAT9050.16375881673850343No Hit
GTACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA8980.1624921739571006No Hit
GTTTCGCTCGCAGCCGGTCTGGAGCGAAACTTATTGGGACTGACAACTCC8860.1603207863318387No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8860.1603207863318387No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC8850.16013983736306686No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8730.15796844973780494No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8480.15344472551850927No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA8470.15326377654973744No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8460.1530828275809656No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8450.1529018786121938No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC8400.15199713376833465No Hit
TGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG8390.15181618479956283No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8360.15127333789324734No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA8250.14928289923675725No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8250.14928289923675725No Hit
CCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATT8180.14801625645535446No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC8140.14729246058026715No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC8100.14656866470517985No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8020.14512107295500523No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7820.1415020935795687No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCG7680.1389688080167631No Hit
ATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAG7670.1387878590479913No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG7640.1382450121416758No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7620.13788311420413216No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7600.13752121626658848No Hit
CTCCAAAAGACACCAAATACTCAAGCACAGTTTCTCTTCCAAAAGTAAGA7570.13697836936027302No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT7500.13571172657887023No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTGCT7400.13390223689115197No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG7390.13372128792238014No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATA7380.1335403389536083No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7350.13299749204729283No Hit
CAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCA7290.13191179823466187No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7260.13136895132834636No Hit
TCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCT7240.13100705339080274No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT7180.12992135957817175No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT7160.1295594616406281No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7100.12847376782799713No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7050.12756902298413803No Hit
GTATCCAGTTGGCAGCACACCCGAGCAGCCATGGGAAGGAGGTGTACTTC7030.12720712504659437No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6990.12648332917150704No Hit
GTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAA6980.12630238020273524No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6910.12503573742133242No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATT6840.12376909463992965No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6720.12159770701466774No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6650.12033106423326492No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC6630.1199691662957213No Hit
GTGTAAACAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC6600.1194263193894058No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6570.11888347248309031No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCAC6550.11852157454554665No Hit
AAACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAA6540.11834062557677484No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATC6520.11797872763923119No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6480.11725493176414387No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG6450.1167120848578284No Hit
GCCATGGGAAGGAGGTGTACTTCCGAGAGAGGACAACGGAGTTGTCAGTC6450.1167120848578284No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6440.11653113588905657No Hit
ATCAGCGCCTGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACCGCG6400.11580734001396925No Hit
GGTCCTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6350.11490259517011013No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC6340.1147216462013383No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTCTACGTCCCGTCGGCGCTGAAT6310.11417879929502281No Hit
ATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTG6250.11309310548239186No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6090.11019792198204263No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6050.10947412610695531No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC6010.10875033023186802No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5970.1080265343567807No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5960.10784558538800887No Hit
ACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA5960.10784558538800887No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5930.1073027384816934No Hit
TCAACTACCAGCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGG5920.10712178951292158No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGGGC5900.10675989157537791No Hit
TAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAG5890.10657894260660608No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGCGGA5870.10621704466906243No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5790.10476945291888781No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5730.10368375910625685No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC5720.10350281013748502No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5710.1033218611687132No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5690.10295996323116954No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG5680.1027790142623977No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5660.10241711632485408No Hit
AGTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5630.10187426941853858No Hit
TGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5600.10133142251222309No Hit
CGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGT5590.10115047354345129No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5580.10096952457467946No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5570.10078857560590762No Hit
TATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGT5560.1006076266371358No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA5530.10006477973082031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)