FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552642
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG21340.38614509935907876No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16280.2945849211605343No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA16100.2913278397226414No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG15400.27866141190861354No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14760.2670806779072166No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG14200.25694753565599426No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13780.2493476789675776No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13070.23650030218477786No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA12830.232157526934254No Hit
GGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTT12800.2316146800279385No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12400.22437672127706543No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12070.21840540530759517No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12060.2182244563388233No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT12010.21731971149496418No Hit
GGTTGTAGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGT11970.21659591561987687No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11830.2140626300570713No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG11690.21152934449426572No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11640.21062459965040659No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC11170.20211999811813072No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG11150.20175810018058707No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11140.20157715121181524No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10610.19198685586690842No Hit
GGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGT10550.19090116205427746No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9730.17606334661498765No Hit
GTTTCGCTCGCAGCCGGTCTGGAGCGAAACTTATTGGGACTGACAACTCC9560.1729872141458666No Hit
TCTACAACCAGCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGG9340.16900633683288638No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9210.16665400023885266No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9200.1664730512700808No Hit
ATTGACAACAGTGCCAGCAGCCCCTCCTCCTGCCTCCACCAATCGGCAGT9180.16611115333253715No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9160.1657492553949935No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC9120.1650254595199062No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9020.16321596983218792No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG9010.1630350208634161No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA8670.15688275592517398No Hit
GTTTTGTGCCCATATTAACATTGACATAGCCGACTACTAATTCCCTGGAT8650.15652085798763032No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8620.15597801108131484No Hit
TTCGTAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGA8480.15344472551850927No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG8430.15253998067465013No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8430.15253998067465013No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8230.1489210012992136No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8180.14801625645535446No Hit
ATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAG8160.1476543585178108No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8030.14530202192377706No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA8020.14512107295500523No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7970.1442163281111461No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7900.1429496853297433No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC7880.14258778739219966No Hit
CAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCA7830.14168304254834052No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCG7790.1409592466732532No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7680.1389688080167631No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT7620.13788311420413216No Hit
CTCCAAAAGACACCAAATACTCAAGCACAGTTTCTCTTCCAAAAGTAAGA7470.13516887967255475No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7350.13299749204729283No Hit
ATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTG7340.13281654307852098No Hit
CCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATT7230.1308261044220309No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG7230.1308261044220309No Hit
GTACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7220.13064515545325908No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC7170.12974041060939992No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7140.12919756370308447No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7120.1288356657655408No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTGCT7120.1288356657655408No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT7090.1282928188592253No Hit
GTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAA7070.12793092092168165No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATACTGCGCGGGACGTCC7010.1268452271090507No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7010.1268452271090507No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6960.12594048226519158No Hit
GTGTAAACAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC6940.12557858432764793No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATC6910.12503573742133242No Hit
TGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6830.12358814567115782No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6830.12358814567115782No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG6790.12286434979607051No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6780.12268340082729869No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG6700.12123580907712407No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6690.12105486010835224No Hit
TCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCT6660.12051201320203676No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6580.11906442145186215No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6560.11870252351431848No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6510.11779777867045936No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6480.11725493176414387No Hit
GTATCCAGTTGGCAGCACACCCGAGCAGCCATGGGAAGGAGGTGTACTTC6460.11689303382660021No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATA6320.11435974826379464No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTCTACGTCCCGTCGGCGCTGAAT6320.11435974826379464No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6310.11417879929502281No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGCGGA6280.11363595238870734No Hit
ATCAGCGCCTGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACCGCG6190.1120074116697609No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6180.11182646270098907No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6180.11182646270098907No Hit
GTACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6180.11182646270098907No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6140.11110266682590175No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCAC6140.11110266682590175No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT6130.11092171785712993No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATT6100.11037887095081446No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6040.10929317713818348No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6000.10856938126309619No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5990.10838843229432435No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5980.10820748332555252No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA5980.10820748332555252No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5950.10766463641923704No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5940.10748368745046522No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5740.10386470807502868No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC5710.1033218611687132No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5680.1027790142623977No Hit
TATATGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGT5600.10133142251222309No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5590.10115047354345129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)