FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689914
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63110.9147516936893583No Hit
CATATAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT17610.2552492049733735No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT17450.2529300753427239No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT15260.22118698852320726No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12670.18364607762706656No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12440.18031232878300774No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12440.18031232878300774No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12350.17900781836576735No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12330.17871792716193613No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG12260.17770330794852693No Hit
GTGTGGCTATCCTGCCTTGAAGCCTTTATATGCATGCATACAATCTAAGC12110.1755291239197929No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11840.17161559266807166No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11540.16726722461060364No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT11380.16494809497995402No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10690.15494684844777754No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATA10020.14523549311943226No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9670.14016239705238623No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9480.1374084306159898No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT9400.136248865800665No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT9270.1343645729757622No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGAAGCCTTTATATGCATGCATA8840.12813191209339134No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8660.12552289125891053No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8500.12320376162826092No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT8430.1221891424148517No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG7980.11566659032864966No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC7950.11523175352290285No Hit
GTTTATATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7940.11508680792098726No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7930.11494186231907166No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT7880.11421713430949365No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7830.11349240629991565No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTCTTTGATTTTTAGTACAATA7530.10914403824244762No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA7160.10378105097157038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)